人卵巢癌组织中C-myc基因的扩增

Amplification of proto-oncogene C-myc in human ovarian carcinoma.

采用聚合酶链反应(PCR)及激光扫描技术,对1期4例、Ⅱ期12例、Ⅲ期18例、Ⅳ期4例的卵巢癌组织及6例正常卵巢组织的 DNA 作 C-myc 基因扩增检测,分析不同临床分期、病理分化程度及淋巴结转移情况与 C-myc 基因扩增率之间相关性。结果,38例卵巢癌 C-myc 扩增率47%,癌旁组织40%,淋巴结转移的10例全部扩增,正常组织未有扩增.提示:1.C-myc 扩增与临床分期、病理分化程度、淋巴结转移密切相关。2.PCR-激光扫描技术与其它各种分子杂交技术相比,具有简单、快速、特异、没有放射性污染等优点,适合于临床应用。

For to observe the amplification rate of C-myc in human ovarian carcinoma,using PCR and laser- scanning techniques,the amplification rates of C- myc were testing in 4 stage Ⅰ,12 stage Ⅱ,18 stage Ⅲ,4 stage Ⅳ ovarian carcinoma and 6 no- mal ovarian tissues.The correlations among clini- cal stage,pathological differentiation,lymph node metastasis and amplification rate of C-myc were analysed.Results: The amplification rate of C- myc in 38 ovarian carcinoma,paracarcinoma,10 metastasized lymph node and normal ovarian tis- sues was 47%,40%,100% and 0 respectively. Conclusions: (1)There were close correlations be- tween amplification rate of C-myc and clinical stage,pathological defferentiation,lymph node metastasis.(2)The goodness of simplicity,rapidi- ty,differentiation,no radioactive pollution of PCR-Laser scanning techniqueas comparing with other mlecular hybridization techniques make it suitalbe for clinical testing.