哮喘发作期患者血清MIP-1α表达水平的研究

The investigation of macrophage inflammatory protein-1alpha in serum from period of onset asthmatic patients

目的研究巨噬细胞炎症蛋白-1α(MIP-1α)在哮喘发作期患者的血清表达水平,以及其与哮喘严重度的关系。方法:选取哮喘发作期患者50例、哮喘缓解期患者20例、健康对照者20例,采用双抗体夹心酶联免疫吸附法(ABC-ELISA)测定血清MIP-1α浓度,同时测外周血嗜酸性粒细胞计数(EOS)、第一秒用力呼气容积占预计值百分比(FEV1占预计值%)。结果:哮喘发作组血清MIP-1α表达水平明显高于哮喘缓解组及健康对照组,(P<0.05)。哮喘发作期患者血清MIP-1α表达水平与外周血EOS计数之间存在明显的相关关系(r=0.548,P<0.05)。哮喘发作期患者血清MIP-1α表达水平与FEV1占预计值%之间存在负相关(r=-0.251,P<0.05)。结论哮喘发作期血清MIP-1α表达水平明显增高,与外周血EOS计数和FEV1占预计值%之间存在明显的相关关系。

Objective Investigate the level of macrophage inflammatory protein-1 alpha （ MIP-1α ） in serum from period onset asthmatic patients, and evaluate the relation between MIP-1α and the severity of asthma. Method Collect 50 period of onset asthmatic patients ,20 paracmastic asthmatic patients and 20 healthy individuals. The levels of MIP-1α were measured by enzyme link immunoabsorbent assay（ELISA） ,while the number of eosinophilic granulocyte（EOS） and the percentage of FEV1 in prediction（ FEV1% ） were also measured. Results The levels of MIP-1α were significantly higher in asthmatic patients that in period of onset than that in patients of paracmastic asthma and healthy individuals（ P 〈0.05 respectively）. The level of MIP-1α in period of onset asthma was closed correlaled with EOS and negatively correlated with FEV1% （ r = 0. 548, P 〈 0. 05 r = - 0. 251, P 〈 0. 05 respectively）. Conclusion The level of MIP-1α was significantly heightened in period of onset asthma, and was colsedly correlated with EOS and FEV1%.