加味丹参饮对大鼠实验性心肌缺血损伤的抗凝血作用研究

Anticoagulation Effects of Supplemented Salvia Beverage on Experimental Myocardial Ischemia in Rats

目的研究加味丹参饮对大鼠实验性心肌缺血损伤的抗凝血作用。方法采用结扎冠状动脉左降支的大鼠心肌缺血模型,观察加味丹参饮高、中、低剂量(8.24、4.12、2.06g/kg)对造模后大鼠活化凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)、血小板粘附率、全血血小板聚集、血栓形成长度和血栓干、湿重以及纤维蛋白原(Fg)含量的影响。结果与造模组比较,加味丹参饮高剂量组对PT和TT缩短、纤维蛋白原含量降低均有显著的抑制作用(P<0.05);加味丹参饮高、中剂量组能明显抑制二磷酸腺苷(ADP)和胶原诱导的血小板聚集(P<0.01,P<0.05);加味丹参饮高、中、低剂量组均能显著降低血栓形成长度和干、湿重(P<0.01,P<0.05)。结论加味丹参饮对大鼠实验性心肌缺血具有显著保护作用,可能与其抗凝血作用密切相关。

Objective To study the anticoagulation effects of Supplemented Salvia Beverage （SSB） on myocardial ischemia in rats. Methods Rat myocardial ischemia model was induced by occluding the left anterior descending coronary artery, the effects of SSB （8.24, 4.12, 2.06 g/kg） on the activated partial thromboplastin time （APTT）, prothrombin time （PT）, thrombin Time （TT）, platelet adherence rate, platelet aggregation, length of thrombus and the dry and wet weight of thrombus, as well as amount of Fibrinogen （Fg） were observed. Results Compared with model group, the shortening of PT and TT, and the decrease of Fg were obviously suppressed in high dosage SSB group （P〈0.05）. The platelet aggregation induced by Adenosine Dimonophosphate （ADP） and collagen was suppressed significantly in high and middle dosage SSB group respectively （P〈0.01, P〈0.05）. The length of thrombus was shortened and the dry and wet weight of thrombus were lightened obviously in all dosage groups （P〈0.01, P 〈0.05）. Conclusion Anticoagulation effects of Supplemented Salvia Beverage may play a key role in the myocardial protection.