内源性硫化氢和胱硫醚-β-合成酶mRNA在支气管哮喘大鼠中的表达及意义

Regulatory effect of endogenous hydrogen sulfide and cystathionine-β-synthase on acute asthma rats

目的观察支气管哮喘（简称哮喘）大鼠胱硫醚-β-合成酶（cystathionine-β-synthase，CBS）mRNA及硫化氢（hydrogensulfide，H。S）的表达变化，探讨CBS与Hzs的相关性。方法采用哮喘大鼠模型，将30只SD大鼠随机分成哮喘组、对照组、地塞米松组，分光光度法测定血浆H2S含量，RT—PCR法检测肺组织CBSmRNA的表达水平。结果血浆中H2S含量哮喘组显著低于对照组，地塞米松组与哮喘组、对照组相比差异无统计学意义。肺组织CBSmRNA的表达水平哮喘组、地塞米松组显著低于对照组，地塞米松组显著高于哮喘组。肺组织CBSmRNA和血浆中H2S的表达水平呈显著正相关。结论哮喘大鼠血浆中H2S的含量和肺组织CBSmRNA的表达下降，两者呈正相关，提示可能参与了哮喘的炎症过程。地塞米松改善哮喘炎症可能部分通过H2S／CBS体系而起作用。

Objective To study the expressions of endogenous hydrogen sulfide （H2S） and cystathionine-β- synthase（CBS） mRNA on acute bronchial asthma（asthma）, and to investigate the correlation of CBS and H2 S. Methods Thirty male SD rats were randomly divided into asthma group,control group,and treated group with dexamethasone （DXM）. The content of H2S was detected in the plasma by spectrophotometry. The expression of CBS mRNA in the lung tissues was detected by RT-PCR. Results Compared with that of control group, the content of H2S of asthma group in the plasma significantly decreased;and there was no significance between treated group with DXM and control group, treated group with DXM and asthma group. Compared with that of control group,the expression of CBS mRNA of asthma group and treated group with DXM significantly decreased. Compared with that of asthma group, the expression of CBS mRNA of treated group with DXM significantly increased. There was significantly positive correlation between the expression of CBS mRNA in the lung tissues and the content of H2S in the plasma. Conclusions The expression of CBS mRNA in the lung tissues and the content of H2S in the plasma of asthmatic rats show decrease, there is correlation between them. They possibly participate in the inflammation process of asthma. DXM reducing the airway inflammation of asthma is possibly partly through the system of H2S/CBS.