荷瘤小鼠虚证、实证垂体基因表达的差异

Difference of Genes Expression in Pituitary of Tumour Mice with Sthenia Syndrome and Asthenia Syndrome

目的:揭示荷瘤小鼠实证与虚证垂体基因表达的差异。方法:采用四诊计量检测方法及计量辨证方法,运用GeneChip Mouse Exon 1.0 STArray等技术,检测H22荷瘤小鼠早期邪毒壅盛证和气虚证、中期阳气虚证、中晚期气阴阳虚证共3阶段4个证候垂体基因表达的差异,重点关注那些表达量大、差异显著的基因。筛选出邪毒壅盛证较其他三个证候一致上调或下调的基因。结果:筛选出邪毒壅盛较其他三证一致上调的基因121个,介绍了邪毒壅盛证芯片读数大于1500的9个基因:Gabra6、D0H4S114、Alas2、Pvalb、Plp1I、tpr1、Ndrg2、LOC627016、Gpm6b;一致下调的基因340个,介绍了正常小鼠芯片读数大于1500的21个基因:Abpg、Pip、Dlk1、Car6、Eif3s5、Gnb2、Pcsk1n、Aplp1、Slc22a17、Dnpep、Rnf187、LOC639100、Rab1b、Aldh2、Pten、Tapbp、2310044H10Rik、Cxxc5、Gatad1、Ren1、Lrp11。结论:H22荷瘤小鼠的实证与虚证,其垂体基因存在大量的表达差异,其中部分可能是实证与虚证的标志基因。

Objective： To reveal the difference of genes expression in pituitary of H22 tumour mice with sthenia syndrome and asthenia syndrome. Methods： By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed pituitary gene expression about poisonous pathogenic factors syndrome and asthenia of qi syndrome on earlier period, asthenia of yang and qi syndrome on intermediate stage, asthenia of qi and yin and yang syndrome on advanced stage with H22 tumour mice. Genes expressed highly and differentially distinguished are analyzed on the paper. Screen up-regulated coincident and down-regulated coincident genes in poisonous pathogenic factors syndrome compared to other three syndromes. Resuhs： 121 up-regulated coincident genes were obtained, 9 genes whose array indication exceeded 1500 in poisonous pathogenic factors syndrome were introduced： Gabra6, DOH4S114, Alas2, Pvalb, Plpl, ltprl, Ndrg2, LOC627016, Gpm6b; then 340 down-regulated coincident genes were obtained, 21 genes whose array indication exceeded 1500 in normal mice： Abpg, Pip, Dlk1, Car6, Eif3s5, Gnb2, Pcskln, Aplpl, Slc22a17, Dnpep, Rnf187, LOC639100, Rablb, Aid-h2, Ptcn, Tapbp, 2310044H10Rik, Cxxc5, Gatadl, Renl, Lrpll. Conclusion： After the tumorigenesis, gene expression change is distinguished in pituitary of H22 tumour mice with sthenia syndrome and asthenia syndrome. Some are passible the marker genes of sthenia syndrome and esthenia syndrome.