期刊文献+

粤北翁源蔗区甘蔗宿根矮化病调查 被引量:8

Survey of sugarcane ratoon stunting disease in Wengyuan sugarcane regions of northern Guangdong
下载PDF
导出
摘要 为明确粤北翁源蔗区甘蔗宿根矮化病(Ratoon Stunting Disease,RSD)的发生状况,给甘蔗健康种苗生产提供科学依据,本研究通过斑点酶链免疫试验(DB-EIA),对随机采自翁源蔗区的232个蔗茎样品进行检测。结果表明:在232个蔗茎样品中,RSD检出率为28.4%;主要栽培品种新台糖22号、粤糖83-271、粤糖93-159、新台糖16号、粤糖85-177、黑皮果蔗的RSD检出率分别为3.2%、54.8%、54.2%、17.9%、50.0%和100.0%;坝仔、周陂、庙墩、龙仙等镇的甘蔗RSD发生较重,检出率分别为37.5%、36.4%、34.4%和30.4%;新植蔗的RSD检出率为35.6%,宿根蔗的RSD检出率为26.0%,田块的RSD检出率达73.5%,说明甘蔗宿根矮化病在翁源蔗区已普遍发生,且主栽品种感病严重。 Sugarcane ratoon stunting disease(RSD) is thought as an economically important disease of sugarcane in most countries where the crop is grown. In order to determine RSD infection levels and provide information for developing healthy seedcane in Wengyuan sugarcane regions,232 stalk samples were randomly taken from sugarcane fields in this region and tested using Dot Blot Enzyme linked lmmuno Assay (DB-EIA). The average percentage of infected stalks was 28.4% in all 232 stalk Samples. There were higher RSD infection rates in four towns included to Bazhi, Zhoubie, Miaoden and Nongxian, which were 37.5%,36.4% ,34.4% and 30.4%,respectively. The average stalk infection levels in major cultivations ROC22, YT83-271, ROC16,YT85-177 and Badila (fruit cane) were 3.2%,54.8%,54.2%,19.7%,50.0% and 100.0%,respectively.In addition, the average percentage of infected stalks in plant and ratoon crop were 35.6% and 26.0%,respectively,and incidence of all surveyed field-blocks was 73.5%.h was concluded that widespread occurrence of RSD was in Wengyuan sugarcane regions, and higher incidence of RSD was on major commercial varieties.
出处 《广东农业科学》 CAS CSCD 北大核心 2009年第1期60-62,共3页 Guangdong Agricultural Sciences
基金 广东省科技计划项目(2006A20207001) 农业部"948"项目(2006-G37) 科技部科研院所技术开发研究专项资金项目(2008EG111203)
关键词 甘蔗 宿根矮化病 DB—EIA sugarcane ratoon stunting disease Dot Blot Enzyme linked Immuno Assay(DB-EIA)
  • 相关文献

参考文献6

二级参考文献20

  • 1邓展云,王伯辉,刘海斌,朱秋珍,李鸣,王维赞,谭裕模.广西甘蔗宿根矮化病的发生及病原检测[J].中国糖料,2004,26(3):35-38. 被引量:54
  • 2Glyn James. A review of ratoon stunting disease[J]. Sugar Cane, 1997,(4):9-14.
  • 3CW迪芬巴赫 G S 德维克斯勒.PCR技术实验指南PCR[M].北京:科学出版社,2002..
  • 4黄孟群 肖镇杰.广东宿根矮化病调查报告[J].甘蔗糖业,1987,(2):39-40.
  • 5Gillaspie, A. G. etal. Ratoon stunting disease. Disease of Sugarcane. εleavicr Amsterdam-Oxford-New York-Tokyo. 1989.
  • 6Davis, M:J. atel. Ratoon stunting disease of sugarcane: Isolation of the causal bacterium. Science. 240:1365-1367.
  • 7马丁 J P,阿伯特 E V,休兹 C G(陈庆龙译).世界甘蔗病害(第一卷)[M].北京:农业出版社,1982.
  • 8MATTHEWS A. New method makes identification of RSD more efficient[J]. BSES Bulletin, 1993 (44) :16 - 17.
  • 9CROFT B J. A method for rating sugarcane culfivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay[J].Australian Plant Pathology,2002,31(1) :63 - 66.
  • 10CROFT B J, GREET A D, LEAMAN T M, et al. RSD diagnosis and varietal resistance screening in sugar cane using the EB-EIA technique [C]//Procedure Australian Sociate Sugar Cane Technology, 1994:143- 151.

共引文献80

同被引文献85

  • 1古丽尼沙·沙依木,张志东,杨波,章世奎,唐琦勇,宋素琴,朱静,郭春苗,顾美英.不同品种苹果树内生细菌群落多样性及功能[J].微生物学通报,2020,47(2):500-511. 被引量:12
  • 2邓展云,王伯辉,刘海斌,朱秋珍,李鸣,王维赞,谭裕模.广西甘蔗宿根矮化病的发生及病原检测[J].中国糖料,2004,26(3):35-38. 被引量:54
  • 3沈万宽,邓海华,周国辉,李奇伟.应用DB-EIA技术快速检测甘蔗宿根矮化病研究[J].植物保护,2007,33(2):110-113. 被引量:14
  • 4沈万宽,郑学文,陈仲华,刘培林,邓海华.湛江农垦蔗区甘蔗宿根矮化病调查研究[J].中国农学通报,2007,23(4):387-391. 被引量:22
  • 5陈庆龙.世界甘蔗病害[M].北京:中国农业出版社.1982.
  • 6MCKERN N M, SHUKLA D D, TOLER R D, et al. Confirmation that the sugarcane mosaic virus subgroup consists of four distinct po- tyvirus by using peptide profiles of coat proteins[ J ]. Phytopathology, 1991,81 : 1025 - 1029.
  • 7SHUKLA D D, FRENKEL M J, MCKERN N M, et 81. Present sta- res of the sugarcane mosaic subgroup of potyvirus [ J ]. Arch Virol Supp, 1992, 15:363-373.
  • 8WANG Z K, COMSTOCK J C, HATZILOUKAS E, et al. Compari- son of PCR Bio-PCR, DIA ELISA and isolation on semiseleetive me- dium for detection of Xanthomonas albilineans, the causal agent of leaf scald of sugarcane[ J]. Plant Disease, 1999, 48 : 245 -252.
  • 9ROTT P, DAVIS M J , BAUDIN P. Serological variability in Xan- thomonas albilineans causal agent of leaf scald disease of sugarcane [J]. Plant Pathology, 1994, 43:344 -349.
  • 10ASTUA-MONGE G, STALL R E, DAVIS M J. Diagnosis of ratoon stunting disease of sugarcane by PCR-based procedure [ J ]. Sugar Azucar, 1995, 90(6) : 37.

引证文献8

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部