摘要
为明确粤北翁源蔗区甘蔗宿根矮化病(Ratoon Stunting Disease,RSD)的发生状况,给甘蔗健康种苗生产提供科学依据,本研究通过斑点酶链免疫试验(DB-EIA),对随机采自翁源蔗区的232个蔗茎样品进行检测。结果表明:在232个蔗茎样品中,RSD检出率为28.4%;主要栽培品种新台糖22号、粤糖83-271、粤糖93-159、新台糖16号、粤糖85-177、黑皮果蔗的RSD检出率分别为3.2%、54.8%、54.2%、17.9%、50.0%和100.0%;坝仔、周陂、庙墩、龙仙等镇的甘蔗RSD发生较重,检出率分别为37.5%、36.4%、34.4%和30.4%;新植蔗的RSD检出率为35.6%,宿根蔗的RSD检出率为26.0%,田块的RSD检出率达73.5%,说明甘蔗宿根矮化病在翁源蔗区已普遍发生,且主栽品种感病严重。
Sugarcane ratoon stunting disease(RSD) is thought as an economically important disease of sugarcane in most countries where the crop is grown. In order to determine RSD infection levels and provide information for developing healthy seedcane in Wengyuan sugarcane regions,232 stalk samples were randomly taken from sugarcane fields in this region and tested using Dot Blot Enzyme linked lmmuno Assay (DB-EIA). The average percentage of infected stalks was 28.4% in all 232 stalk Samples. There were higher RSD infection rates in four towns included to Bazhi, Zhoubie, Miaoden and Nongxian, which were 37.5%,36.4% ,34.4% and 30.4%,respectively. The average stalk infection levels in major cultivations ROC22, YT83-271, ROC16,YT85-177 and Badila (fruit cane) were 3.2%,54.8%,54.2%,19.7%,50.0% and 100.0%,respectively.In addition, the average percentage of infected stalks in plant and ratoon crop were 35.6% and 26.0%,respectively,and incidence of all surveyed field-blocks was 73.5%.h was concluded that widespread occurrence of RSD was in Wengyuan sugarcane regions, and higher incidence of RSD was on major commercial varieties.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第1期60-62,共3页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2006A20207001)
农业部"948"项目(2006-G37)
科技部科研院所技术开发研究专项资金项目(2008EG111203)
关键词
甘蔗
宿根矮化病
DB—EIA
sugarcane
ratoon stunting disease
Dot Blot Enzyme linked Immuno Assay(DB-EIA)