摘要
在pH为2.60的B—R缓冲介质中,变色酸2R与人血清白蛋白形成复合物,导致变色酸2R退色,吸光度的降低与人血清白蛋白的浓度成正比。线性范围为0~60ug/mL,灵敏度ε=5.53×10^5L·mol^-1·cm^-1。用于人血清白蛋白的测定,方法稳定,手续简便,结果满意。
A novel method was described for the spectrophotometric determination of human serum albumin ( HSA ), which was based on a fading reaction between ehromotrope 2R and HAS to form complexes in a B-R buffer at a pH value of 2.6.The decrease in absorbance was proportional to the concentration of HAS, The linear ranges were between 0 - 60 ,ug/mL and the molar absorptivity ( ε ) was 5.53 × 10^5 L·mol^- 1. cm^- 1. The method has been used for the determination of HSA with satisfactory results.
出处
《化学试剂》
CAS
CSCD
北大核心
2009年第1期39-40,54,共3页
Chemical Reagents
基金
河南省自然科学基金项目(0411021600)
河南省教育厅自然科学基金项目(200510482009)