鲨鱼肝再生因子对大鼠酒精性肝损伤的保护作用

Protective Effect of Shark Hepatic Regenerative Factor on Alcoholic Liver Injury in Rats

为探索鲨鱼肝再生因子(sHRF)对大鼠慢性酒精性肝损伤的保护作用,采用白酒灌胃法建立大鼠酒精性肝病模型,给药5周后取血清及肝组织测定各项肝指标,结果表明sHRF能显著降低酒精所致的大鼠血清ALT、AST的异常升高,也能抑制酒精损伤后大鼠肝组织中甘油三酯含量的升高,此外,sHRF能在一定程度上增加白蛋白含量,但对总蛋白含量无明显影响,sHRF给药治疗组还可明显提高SOD活性,降低MDA含量,提高大鼠清除体内氧自由基能力,减少过氧化脂质对组织器官的损伤,组织切片发现各给药组肝脏总体病变程度较模型组轻,说明sHRF对大鼠慢性酒精性肝损伤有一定的防护作用。

To study the protective effect of shark Hepatic Regenerative Factor （sHRF） on alcoholic liver injury in rats, alcoholic liver disease of animal model was established by ig. alcohol. The activities of Serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST, the contents of albumin, total protein, malondialdehyde（MDA）, triglycerides（TG） and Superoxide Dismutase （SOD） in liver tissue were measured after 5 weeks. The results showed that sHRF could not only create a prominent prevention to the abnomal increase in ALT and AST in rats＇ serum induced by alcohol, but also restrain the increase of triglyceride in the hepatic tissue of rats. In addition, sHRF could lead albumin content to increase, but the content of total protein was without obvious change. Besides, sHRF administrating group could apparently enhance the activity of SOD, reduce the content of MDA, improve the rats＇ eliminating ability of oxygen free radicals in the body, as well as reduce the injury of lipid peroxide to tissues and organs. Tissue slice displayed that the hepatic pathological changes of the administrating group are less severe than those of the model group. It was concluded that sHRF had significant protective effect on alcoholic liver injury in rats.