小麦条锈菌条中32号生理小种SCAR检测标记的建立

Establishment of scar detection marker of Puccinia striiformis f.sp. tritici race CY32 in China

【目的】建立具有应用价值的小麦条锈菌条中32生理小种的SCAR检测标记。【方法】用100条随机引物对我国目前主要流行的12个小麦条锈菌生理小种进行RAPD筛选,寻找特异性片断,并对其进行克隆、测序,将该特异性片段转化成条中32号小种的SCAR专化型标记。设计并合成SCAR特异性引物,对不同来源的条中32号进行SCAR检测。【结果】引物S1271从条中32号生理小种中扩增出长度为320 bp的特异片段,该片段可作为条中32号的SCAR标记。从不同来源的条中32号生理小种中扩增出了筛选到的SCAR标记。【结论】成功建立了条中32专化SCAR标记。

[Objective] The research was to estiblish a useful SCAR marker of CY32 examination about strip rust. [Method] The special fragment of 12 strip rust races from 100 random primers in China were sifted and cloned. The sequence results were tested and design scar marker was made. Special PCR primer of scar was designed,and CY32 from different areas about scar was tested. [Result] A special fragment of the length is 320 bp by RAPD sifting from primer S1271. It can be used as the CY32 SCAR marker. SCAR marker from different CY32 was amplified. [Conclusion] Special SCAR marker about CY32 was established successfully.