回归正交试验法优化菜芙蓉茎中总黄酮的提取工艺

Optimization on extraction methods of flavonoids in stalk of Hibiscus manihot with orthogonal regression

研究了菜芙蓉茎中黄酮类物质的分离提取方法,用回归正交试验法优化了提取条件,采用吸光值法、薄层层析法和高效液相色谱法定性定量分析了提取液。优化的提取条件为:乙醇体积分数69%,提取温度61.5℃,料液比为1∶41.5(mg/mL),提取时间4.8 h。此条件下黄酮类物质的提取率为1.023 mg/g。

The extracting condition on flavonoids from Hibiscus manihot was optimized by orthogonal regression experimental design methods. The extract was quantitatively and qualitatively analyse by absorption, TLC method and HPLC, The optimum extracting conditions were 69% alcohol as solvent, extracting temperature 61.5 ℃, stalk material to solvent ratio 1 ： 41.5, extracting time 4.8 h. The maximal extracting rate of flavonoids was 1. 023 mg/g of dry weight.