失血性休克对脾脏细胞因子、免疫球蛋白和补体生成的影响

INFLUENCE OF HEMORRHAGIC SHOCK ON CYTOKINE IMMUNOLOBULIN AND COMPLEMENMT IN SPLEEN

目的:探讨失血性休克对脾脏免疫功能的影响及液体复苏对脾免疫功能的保护作用。方法:健康家犬按weiggers法复制失血性休克模型,观察休克后脾动静脉血与脾组织匀浆中7种细胞因子、3种免疫球蛋白和2种补体的变化以及高氧液复苏后的免疫调整作用。结果:休克组动物动脉血浆中IL-1、IL-6、IL-8、TNF均显著降低,TXB2和6KPGF1a含量显著增高,IgG明显降低。静脉血浆中IL-1、IL-2、IL-6、IL-8、TNF、6KPGF1a均明显低于对照组,免疫球蛋白IgG降低最明显。脾组织匀浆中细胞因子和免疫球蛋白亦有明显改变。结论:休克可引起脾免疫功能降低,高氧液复苏治疗通过促进脾免疫功能恢复而具有免疫调整作用。

Objective：To investigate the immunoregulatory effect of spleen in hemorrhagic shock.Methods： The content of IL-1, IL-2, IL-6, IL-8, TNF, TXB2, 6KPGF1a immunoglobulin A,G,M and complement 3,4 in spleen blood plasma as well as in spleen tissue of dogmodels of hemorrhagic shock before and after treatment were determined by radioimmunoassay.Results： IL-1,IL-6,IL-8,TNF and IgG in arterial blood plasma were reduced obviously,but TXB2 and 6KPGFla were increased obviously; IL-1,IL-2,IL-6,IL-8,TNF,6KPGFla and IgG in venous blood plasm were reduced obviously;the content of eytokines and immunoglobulins in spleen tissue after hemorrhagic shock also varied obviously.Conclusion：The hemorrhagic shock may reduce the function of spleen,but the treatment of hyperoxygenated solution may improve spleen function and immunoregulatory effect.