^(60)Coγ线辐照的油菜花粉致突变活性研究

STUDIES ON MUTAGENIC ACTIVITY OF ^(60)Coγ-RAY IRRADIATED RAPE POLLEN

本文以小鼠骨髓细胞微核率,姐妹染色单体交换率及睾丸细胞减数分裂染色体畸变率为指标检测经2.5kGy^(60)Coγ射线辐照的油菜花粉的致突变性。结果表明:多染性红细胞及有核细胞的微核率,减数分裂染色体畸变率,辐照的及未辐照的花粉和对照组相比均无明显差异。未辐照的花粉诱发的姐妹染色单体交换(SCE)率同对照组相比无明显差异,而灌胃相同剂量3000mg/kg/天×7和6000mg/kg/天×7辐照的花粉同对照组相比,有显著性差异。

The rape pollen contains many biologically active and nutritive su-bstances that are easily spoilt by bacteria because there are abundant nutritive substa-nces. In the present study on disinfection, the rape pollen was irradiated with 2.5kGy ^(60)Coγ-ray. We used micronuciei, sister chromatid exchanges (SCE) of bonemarrow cells and chromosomal aberrations of meiotic cells in mice as an indicaterof chromosomal damage to study the mutagenicity of irradiated rape pollen. The resu-lts are as follows: (1). Micronuclei: The frequency of micronuclei in polychromatic erythrocytes is2. 00‰; nucleated cells is 0. 85‰, in control group. In the numbers of polychromaticcrythrocytes and nucleated cells with micronuclei, there is no obviously difference inirradiated and unirradiated groups. (2) SCE: SCE incidence of control group is 2. 01±0. 12/cell. No significant dif-ference in the frequency of SCE exists between non irradited rape pollen and thecontrol groups. But the frequency of SCE in irradiated rape pollen group (3000 mg/kg/day×7) is 2. 36±0. 12/cell: high dose group (6000 mg/kg/day×7) is 2. 96±0. 14/cell. In comparison with control group, there is significant difference. (3). The meiotic chromosomal aberration: The chromatid breaks, fragments, andunivalents in primary spermatocytes have been obseved. The frequencies of chromoso-mal aberration showed no obviously difference among irradiated and non irradiated rapepollen groups. The univalent frequencies of X-Y chromosomes in the experimentalgroups didn't found the difference with the control group which is 9.2% and ovresimilar to that of the reference.