毛竹种子“以芽繁芽”组培快繁初步研究

A Preliminary Study of Tissue Culture and Propagation of Moso Bamboo by Shoot Proliferation

以毛竹种子为外植体，通过“以芽繁芽”方式找到了较好的外植体消毒技术、增殖培养基、生根培养基和炼苗移栽技术等，初步建立了毛竹种胚外植体“以芽繁芽”组培快繁体系。其中最佳消毒时间为10％次氯酸钠溶液0．1％升汞各20min，消毒成功率可达37％；最佳增殖培养基为MS＋TDZ0．5mg／L＋KT2．0mg／L，增殖4-5代则衰退；生根培养基以1／2MS＋IBA1．0mg／L＋KT0．5mg／L＋AC0．1g／L效果较好；炼苗移植成活率可达69％，可应用生产。

This study optimized the technologies for disinfection of explants, propagation culture medium, rooting medium, seedlings transplanting, etc. by means of shoot proliferation with Moso bamboo seeds as explants, and preliminarily established the tissue culture and propagation system for explants of moso seeds by shoot proliferation. The best disinfection method is to immerse seed explants in 10% sodium hypochlorite for 20 minutes and then in 0.1% mercuric chloride for another 20 minutes, with the rate of disinfection being 37%; the best propagation culture medium is MS ＋TDZ 0.5 mg/L＋ KT2.0 mg/L, but no matter what medium had been used, there would be a recession after four to five subcultures; the best rooting medium is 1/2 MS＋IBA 1.0 mg/L＋ KT0.5 mg/L＋ AC0.1 g/L; seedling transplanting achieves a good result with the survival rate up to 69%, which can be used for production.