蝙蝠葛碱对兔心肌缺血再灌注时血清MDA浓度和SOD活性的影响

Effect of Dauricine on the contents of MDA and the activities of SOD of the serum during myocardial ischemia reperfusion in rabbits

目的探讨蝙蝠葛碱(Dau)对兔心肌缺血再灌注(IR)时血清丙二醛(MDA)浓度和超氧化物歧化酶(SOD)活性的影响。方法24只家兔随机分为假手术对照组、缺血再灌注组、缺血再灌注+蝙蝠葛碱(Dau)干预组各8例。结扎兔左冠状动脉前降支40 min造成心肌缺血再灌注模型,观察缺血前后及再灌注不同时相点血清MDA含量和SOD活性的变化。结果家兔心肌缺血40 min时血清MDA含量开始明显升高(P<0.05),SOD活性开始明显下降(P<0.05)。Dau(剂量3.5 mg/kg)能明显降低心肌缺血40 min及缺血再灌注后兔血清MDA含量,升高血清SOD活性。结论Dau通过减轻兔心肌脂质过氧化作用所造成的损伤,增强SOD活性,提高氧自由基清除能力,对兔心肌缺血再灌注损伤具有一定保护作用。

Objective To explore the effect of Dauricine（Dau） on the contents of MDA and the activities of SOD of the serum during myocardial ischemia reperfusion in rabbits. Methods 24 rabbits were randomly divided into three groups： the control group （n =8）, ischemia reperfusion（IR） group （n =8）, ischemia reperfusion（IR） ＋ Dau intervention group （ n = 8 ）. Left anterior descending coronary artery was ligated to establish the model of myocardial reperfusion injury in rabbits. At 40 minutes after ischemia, the ligation was released to restore blood reperfusion. Evaluate and compare the change of the contents of MDA and the activities of SOD of rabbits serum before and after ischemia and at different times of reperfusion. Results At 40 minutes after ischemia, the content of MDA in rabbits serum was beginning to increase significantly and the activity of SOD was reduced significantly （ both P 〈 0.05 ） compared to the control group. In IR ＋ Dau （ amount of 3.5 mg/kg） intervention group, at 40 minutes after ischemia and the times after reperfusion the corresponding contents of MDA were reduced significantly and the corresponding activities of SOD were increased significantly in rabbits serum compared to the IR group. Conclusion Dau could attenuate the injury induced by lipid peroxidation and enhance the activity of SOD, thus it plays a protective role in myocardial reperfusion injury in rabbits.