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CD147基因发夹结构干扰RNA表达载体的构建及鉴定 被引量:1

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摘要 根据人CD147基因mRNA序列,设计有小发夹结构的3条寡核苷酸序列(shRNA),克隆到空载体pSilencer 4.1-CMV neo中,构建重组质粒,通过测序验证阳性克隆。结果重组质粒测序鉴定与设计的shRNA转录模板序列相同,证实重组质粒构建成功。认为构建CD147 RNA干扰表达载体的成功对未来转移性前列腺癌的基因治疗有重要意义。
出处 《山东医药》 CAS 北大核心 2008年第46期39-40,共2页 Shandong Medical Journal
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参考文献7

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同被引文献14

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