吡格列酮对脂多糖引起的体外培养大脑皮层神经元NO释放的影响

Protection of Pioglitazon on Lipopolysaccharide-induced Insult in the Cultured Cortical Neurons of Neonate Rats

目的探讨吡格列酮（pioglitazon，Pio）能抑制脂多糖（lipopolysaccharide，LPS）引起的培养大脑皮层神经元一氧化氮释放增加，并探讨其抑制作用信号转导机制。方法以体外培养6—7d的乳鼠大脑皮层神经元为研究对象，建立脂多糖损伤和吡格列酮保护模型。采用Griess法测定细胞培养上清液中NO含量。结果脂多糖可导致神经细胞损伤，培养液中NO含量升高；吡格列酮（1umol／L）可明显抑制脂多糖引起的NO含量的升高。结论吡格列酮可明显拮抗脂多糖诱导的神经细胞损伤。

Objective To explore the express effects of pioglitazon （Pio） on lipopolysaceharide - induced NO production to cultured cortical neurons and investigate its signaling pathway. Methods The inflammatory neurotoxicity model was constructed in primary cortical neurons isolated from Sprague - Dawley rats 6 - 7 d neonate rats following a dose lipopolysaccharide to the neurons directly. The contents of NO was detected. Results The cells, exposed to LPS （ 10 ug/mL for 8 h）, showed charateristic change of damage, which could be relieved by pioglitazon （1umol/L） with cell survival rate increment. Pioglitazone could block the increase of NO induced by LPS. Condttsions Pioglitazone can prevent the neurons from the damage of LPS inflammatory neurotoxicity.