组织块法培养兔主动脉平滑肌细胞

The Tissue Explants Adherant Method for Culture of Rabbit Arterial Smooth Muscle Cells

目的探讨培养兔主动脉平滑肌细胞的方法,为心血管疾病的病因机制研究提供有用的体外模型。方法采用组织块贴壁、反转干涸方法培养兔主动脉平滑肌细胞并传代。倒置显微镜下进行形态学观察及免疫细胞化学鉴定。结果成功培养兔血管平滑肌细胞并传代。倒置显微镜下细胞呈典型的"峰-谷"状。免疫细胞化学染色呈棕黄色,均呈阳性表达,胞浆内可见纵行排列肌丝。结论利用组织块贴壁、反转干涸方法成功进行兔主动脉平滑肌细胞原代培养,具有简便易行、培养周期短、纯度高等优点,是心血管疾病良好的体外研究模型。

Objective To discuss the method for primary culture of rabbit arterial smooth muscle cells. To provide useful vitro model for the etiopathogenisis of cardiac vascular diseases. Methods Rabbit arterial smooth muscle cells were cultured with tissue explants adherent and back to roll depletive method and could be passaged. The form of rabbit arterial smooth muscle cells were observed under inverted phase contrast microscope and were identified with the method of immunocytochemistry. Results Rabbit arterial smooth muscle ceils were cultured successfully with tissue explants adherent and back to roll depletive method and could be passaged. Under inverted phase contrast microscope, the growth of the cells showed a typical hill - and - vally pattern. After immunocytochemistry staining, all the cells cytoplasm were stained and positive. Lots of stained buffy actin filament bundles were observed in cell cytoplasm. Conclusions The culture techenique of tissue explants adherent method has the advantages of easy to operate, high efficiency to obtain VSMCs in a shorter cycle time, high purity and so on. They are good vitro model of cardiac vascular diseases.