支气管哮喘淋巴细胞Fas抗原的表达

The expression of Fas antigen on lymphocytes in the patients with asthma

目的探讨哮喘患者淋巴细胞Ｆａｓ抗原的表达状况。方法取哮喘患者外周血淋巴细胞，加入地塞米松１０５ｍｏｌ／Ｌ进行细胞培养，分别于０、２、８、２４小时以流式细胞仪测定淋巴细胞表面的Ｆａｓ（ＣＤ９５）抗原。结果哮喘患者淋巴细胞Ｆａｓ抗原的自发表达较正常对照组低，哮喘组Ｆａｓ为５３％±２０％，正常组为８５％±１８％，（Ｐ＜０．０５）。体外经地塞米松诱导后其Ｆａｓ抗原表达显著增加（Ｐ＜０．０１）。结论哮喘患者免疫亢进可能与免疫细胞Ｆａｓ抗原自发性降低有关。地塞米松可诱导淋巴细胞Ｆａｓ抗原的高表达，可能是地塞米松诱导细胞凋亡的机理之一。

Objective To study the expression of Fas antigen on lymphocytes in patients with asthma. Method The peripheral blood lymphocytes (PBL) isolated from patients with asthma were cultured with dexamethasone 10 5 mol/L, Fas antigen on lymphocytes was detected by flow cytometry at 0, 2 nd, 8 th, 24th hour respectively. Result The native expression of Fas antigen of PBL isolated from the patients with asthma (5 3%± 2.0% , P <0.05) was lower than that from healthy controls (8.5%±1.8%). Increased expression of Fas antigen in the patients with asthma was observed with induction of dexamethasone ( P <0.01). The percentages of Fas positive cell were 5.3%±2.0%, 33%±5%, 63%±11%, 76%±7% at 0, 2nd, 8 th, 24 th hour respectively in asthmatic group. While 8.5%±1.8%, 31%±11%, 49%±10%, 20%±4% respectively in normal control group. Conclusion Decreased native expression of Fas on lymphocytes may be related to airway inflammation in the patients with asthma. Increased expression of Fas induced by Dex may be one of the mechanism of lymphocytes apoptosis.