摘要
目的:探讨头部电针透穴疗法治疗帕金森病的作用机制。方法:以6-羟基多巴胺左侧纹状体微量注射法制备偏侧帕金森病大鼠旋转模型,采用头部电针透穴疗法进行治疗,设立正常组、假手术组、模型组和透针组:①以免疫组织化学方法检测酪氨酸羟化酶阳性细胞的形态、数量;②以RT-PCR技术检测神经干细胞巢蛋白mRNA的表达。结果:①透针组与模型组比较络氨酸羟化酶免疫反应阳性神经元在面密度、数密度及积分光密度方面表达增加(P<0.05);②透针组与模型组比较巢蛋白mRNA表达增加(P<0.05)。结论:头部电针透穴疗法能促进帕金森病模型大鼠黑质内源性神经干细胞的增殖。
Objective To probe into the function mechanism of penetration therapy with head electrical acupuncture on Parkinson＇s disease. Methods Microinjection of 6-hydroxydopamin （6-OHDA） on the left cor- pus striatum was adopted to prepare rotation model of Parkinson^s disease in rat. Penetration therapy with head electrical acupuncture was administered in treatment. Normal group, sham-operation group, model group and penetration therapy group were set up. （1）lmmunohistochemical （IHC） method was used to test the morphology and count of positive cell of tyrosine hydroxylase （TH）. （2）RT-PCR technology was used to detect the expression of nestin mRNA of neural stem cell （NSC）. Results （1）Compared with model group, in pene- tration therapy group, the expressions of TH-positive neurons in immune response were increased in areal density （AD）, numerical density （ND） and integrating optic density （P〈0.05）. （2）Compared with model group, in penetration therapy group, the expression of nestin mRNA was increased （P〈0. 05）. Conclusion Penetration therapy with head electrical acupuncture promotes the proliferation of endogenous neural stem cells in substantia nigra of rat model of Parkinson＇s disease.
基金
the Excellent Discipline Leadership Fund Project of Harbin Science-Technology Administration :2006RFXYS044
