军曹鱼养殖水体及其肠道异养细菌的耐药性研究

Antibiotic resistance of heterotrophic bacteria of digestion guts isolated from cobia(Rachycentron canadum Linnaeus) farming waters

选用常用的15种抗生素,通过纸片扩散法(Kirby-Bauer纸片扩散法,简称K-B法),参照NCCLS抗生素敏感试验操作标准,并以金黄色葡萄球菌Staphylococcus aureusATCC25923和大肠埃希菌Esсheriсhia coliATCC25922为质控菌株,对周年监测分离自养殖军曹鱼水体及肠道的188株优势菌(包含9个菌属)进行了药物敏感性研究。结果显示,抑菌最强的是环丙沙星(耐药率为0),其次是氯霉素、诺氟沙星、庆大霉素、多粘菌素B、复方新诺明,耐药率均小于40%;抑菌效果最差的依次为羧苄青霉素、青霉素G、氨苄青霉素、痢特灵、利福平,耐药率均在40%以上,其中多重耐药的菌株(对4种或4种以上抗生素产生耐药性)共12株,包括水体菌9株和肠道菌3株。

The antibiotic susceptibility tests were carried out by K-B diffusion method on 188 heterotrophie bacteria strains （including 9 genera） isolated for one year both from farming seawater and digestion guts of the eot）ia. 15 different species antibiotics selected were tested to NCCLS performance standards for the antimicrobial susceptibility test, and Staphylococcus aureus ATCC25923 and Escherichia coli ATCC25922 were controlled in the test. The results showed that the most effective antibiotic for the inhibiting growth of the bacteria was Ciprofloxacin （percentage of antibiotic resistance strains was 0） , in the next places were ehloramphenicol, norfloxacin, gentamicin, polymyxin B and compound sulfamethoxazote, as the pereentage of antibiotic resistance strains were less than 40%. The strains resisted of carbenieillin, penicillin G, ampicillin, furazolidone and rifampin was above 40%. It also showed that 12 strains comprised of 9 strains from the farming water and 3 strains from the intestine of eobia resisted to 4 or greater than 4 species antibiotics .