β-胡萝卜素对二甲基亚硝胺致大鼠脂质过氧化的影响

Efficacy of β Carotene on Lipid Peroxidation Induced by N Nitrosodimethylamine in Rats

探讨了给予二甲基亚硝胺（ＮＤＭＮ），同时补充β－胡萝卜素（β－Ｃ）后，大鼠体内抗氧化酶活性和脂质过氧化物含量的变化。结果显示，一定剂量的ＮＤＭＮ可使大鼠血红细胞、肝肾匀浆ＳＯＤ活性和全血ＧＳＨ－Ｐｘ活性下降（Ｐ＜０．０５），血清和肝ＭＤＡ和血清ＲＯＯＨ产生增多（Ｐ＜０．０５）。添加β－Ｃ（２５ｍｇ／ｋｇ）后，ＳＯＤ和ＧＳＨ－Ｐｘ活性均比单纯ＮＤＭＮ组有明显提高（Ｐ＜０．０５）。ＭＤＡ和ＲＯＯＨ含量明显低于ＮＤＭＮ组（Ｐ＜０．０５）。提示自由基和脂质过氧化反应可能也是ＮＤＭＮ及其它亚硝胺致癌的途径之一；一定剂量的β－Ｃ可对抗ＮＤＭＮ引起的自由基和脂质过氧化。

The effect of β carotene (β C) on lipid peroxidation induced by N nitrosodimethylamine (NDMN) in rats was studied. Thirty clear conventional SD rats were randomly devided into 3 groups and were intragastricly given daily water (10ml/kg), NDMN(175mg/kg), and NDMN(175mg/kg) plus β C (25mg/kg) respectively. The results showed that: compared with group 1, the activities of SOD and GSH Px in NDMN group were significantly decreased (P<0.05), the values of MDA and ROOH increased (P<0.05). The activities of SOD and GSH Px in group 3 were significantly higher and the contents of ROOH and MDA were significantly lower than that of NDMN group (P<0.05). These results suggested that free radicals and lipid peroxidation may be a carcinogenic path for NDMN and other nitrosamines. β C had ability to inhibit the lipid peroxidation induced by NDMN.