辐射诱导小鼠免疫细胞蛋白质表达变化及酪氨酸磷酸化

Protein expression and tyrosine phosphorylation in murine immune cells after ionizing radiation

目的：为进一步了解低剂量辐射对免疫细胞早期蛋白质表达及酪氨酸磷酸化的影响。方法：采用SDS-聚丙烯酰胺凝胶电泳、光密度扫描及免疫沉淀方法观察75mGyX射线全身照射对小鼠免疫细胞蛋白质表达变化及酪氨酸磷酸化的影响。结果：照射后小鼠胸腺细胞Mr=28000及Mr=43000蛋白质表达增强；脾细胞Mr=32000及Mr=43000蛋白质表达增强；三种蛋白质均发生酪氨酸磷酸化。结论：这些早期变化的蛋白质参与复杂的细胞功能调节，可能与辐射兴奋效应有关。

Objective: To investigate alterations of protein expression and tyrosine phosphorylation in murine immune cells after low dose radiation. Methods:Change of protein expression in immune cells of mice 0 -12 h after whole they with 75 mGy X-rays were observed with SDS-PAGE followed by densitometricalscanning. Results:It is revealed that a Mr=28 000 protein increased gradually within 12 h and a Mr = 43 000 protein increased in the thymocytes rapidly,reaching a maximum 2 h after irradiation. It was also exhibited that a Mr=43 000 protein alld a Mr=32 000 protein increased in the splenocytes 2 h after irradiation. It is shown that tyrosine phosphorylation of Mr=28 000, Mr=32 000 and Mr=43 000 proteins appeared in the thymocytes after irradiation with immunoprecipitation and western blotting using anti-phosphotyrosine McAb. Conclusion: These findings may have implications in the mechanism of immunoenhancemnt and adaptive response induced by low dose radiation.