羊水淀粉酶同工酶分离测定在判断胎儿成熟度中的意义

Significance of amniotic fluid amylase isozyme in detecting the fetus organ maturity

目的：通过羊水淀粉酶同工酶的分离测定探讨其在判断胎儿成熟度中的意义。方法：采用醋酸纤维膜电泳法对38例不同孕周的羊水中淀粉酶同工酶进行分离，并对其孕周和出生体重关系进行观察。结果：电泳可将羊水淀粉酶分成胰腺淀粉酶同工酶（Ap）和唾液淀粉酶同工酶（As）；随妊娠的进展，Ap变化不大，而As则随妊娠的进展而逐渐增加；Ap／As比值与孕龄和出生体重呈直线负相关；孕周≥37周和出生体重≥2500g者，Ap／As＜0．4的分别为90．6％和91．3％。结论：Ap/As不受羊水量的影响，是判断胎儿成熟度的可靠指标之一。

Objective:To discuss the significance of the amniotic fluid isoamylase (AFI) in detecting the fetus organ maturity by separating and detecting it. Methods: Thirty-eight samples of the AFI in different pregnancy age were separated by the method of the cellulose acetate thin-layer electrophoresis and their relationship with the pregnancy age and the birth weight was also observed. Results: Amniotic fluid amylase could be divided into pancreatic amylase isozyme (Ap) and salivary amylase isozyme (As). Ap had little change dring pregnancy but As increased gradually with the increase of the pregnancy age. Ap/As was adversely relative to the pregnancy age and the birth weight. In the groups that pregnancy age was ≥37 wbs or birth weight ≥2500 g.the percentage of Ap/As<0. 4 was 90. 6% and 91. 3% respectively. Conclusion:Ap/As is not affected by the amount of the amniotic fluid and it is one of the reliable indexes for detecting the fetus organ maturity.