矮牵牛的快速繁殖

Rapid Propagation of Petunia hybrida

矮牵牛带芽嫩茎、嫩叶作为外植体，经培养都能再生完整植株。诱导分化阶段，带芽嫩茎以腋芽发育为主，也有不定芽发育，最适培养基MS＋6-BA0．5mg／L＋NAA0．1mg／L或MS＋6-BA1．0mg／L＋NAA0．5mg／L；叶片以不定芽发育为主，在MS＋6-BA0．5mg／L＋NAA0．5mg／L及MS＋6-BA1．0mg／L＋NAA0．1mg／L培养基上，丛生芽分化效果最好。继代增殖阶段，切割新梢接种于MS培养基或切割丛生芽接种于MS＋6-BA0．5mg／L培养基，均可获得6～10倍的增殖。生根培养阶段，1／2MS＋IBA0．01—0．05mg／L培养基的生根效果好，移栽成活率可达95．6％。

The stems with a bud and the leaves of Petunia hybrida as explants were cultured in tubes and plantlet regeneration was all accomplished. In induction and differentiation stage： The stems with a bud produced axillary buds first, and adventitious buds too. The most appropriate media were MS implementation of 6 - BA0.5mg/L and NAA0. 1mg/L, or 6 - BA1.0mg/L and NAA0.5mg/L; The leaves differentiated adventitious buds. On MS with 6 - BA0.5mg/L and NAA0.5mg/L, or on MS with 6 - BA1.0mg/L and NAA0. 1mg/L, rosette buds grew well. New stems with buds were subcultured on MS, or rosette buds were subcultured on MS with 6 - BAO. 5rag/L, multiplica- Lion rate was 600% - 1000%. Attachment IBA0.01 -0.05mg/L to 1/2MS was benefited to rooting. The regeneration plants were transplant. The survival rate was up to 95.6%.