舌鳞癌组织microRNA表达谱的检测和分析

Detection and analysis on microRNA expression profiles in tongue squamous cell carcinoma

目的探讨不同临床分期的舌鳞癌组织中microRNA分子表达谱的差异,为进一步研究相关microRNA在舌鳞癌发病机制中的作用打下基础。方法分别选取3例早期和3例晚期新鲜舌鳞癌组织及其癌旁舌黏膜为样品,采用高通量的microRNA微阵列筛选不同分期的舌鳞癌组织和癌旁组织中差异表达的内源性microRNA,样品间表达变化的标准以上下2倍作为域值范围。结果在早期舌鳞癌实验组中得到59个有效表达的microRNA分子数据,与配对舌黏膜对照组样品相比具有表达变化的microRNA分子有25个,其中上调表达的有10个,下调表达的有15个;在晚期舌鳞癌实验组中得到40个有效表达的microRNA分子数据,与配对舌黏膜对照组样品相比具有表达变化的microRNA分子有28个,其中上调表达的有26个,下调表达的只有2个。从总体上看,晚期舌鳞癌与舌黏膜间microRNA分子表达差异较大,而早期样品间的表达差异相对较小。结论舌鳞癌组织和癌旁舌黏膜组织中存在差异表达的microRNA分子,不同临床分期的舌鳞癌组织microRNA分子表达谱不同,它们可能分别与舌鳞癌的发生和进展相关,microRNA表达谱有可能成为舌鳞癌分子分期的重要依据。

Objective To study the difference of microRNA expression profiles in tongue squamous cell carcinoma （TSCC） at different clinical stages, for further studies on functions of microRNA in pathogenesis of TSCC. Methods The fresh samples of 3 early stage cases and 3 advanced stage cases of TSCC and corresponding normal tongue tissue （NTT） adjacent to TSCC were obtained, and their endogenous different microRNAs were screened by high-throughput microRNA microarray. Results Fiftynine effective microRNAs expression data were obtained in the test group of early stage TSCC and 25 microRNAs were changed in TSCC compared with that of the control group of NTT, including a set of 10 overexpressed microRNA and 15 downexpressed microRNAs; Forty effective microRNAs expression data were obtained in the test group of advanced stage TSCC and 28 microRNAs were changed in TSCC compared with that in the control group of NTT, including a set of 26 overexpressed microRNAs and 2 downexpressed microRNAs. Additionally, the difference of microRNA expression in the group of advanced stage TSCC was more significant than that in the group of early stage TSCC. Conclusions There are different expressed microRNA between TSCC and NTT, and the microRNA expression profiles are different between early stage TSCC and advanced stage TSCC. These different microRNA might be correlated to the process of carcinogenesis and progression of TSCC. The microRNA expression profiles might be important evidence of molecular stage of TSCC.