摘要
应用RACE-PCR技术,克隆了全长2743 bp的SD-大白鼠催乳素受体基因(gPRLR)cDNA。序列分析表明,cD-NA含421 bp 5′-UTR、1357 bp编码区和218 bp 3′-UTR。比对奶牛催乳素受体基因并将前217 bp看作第1外显子,则gem基因可划分为13个外显子。推导的蛋白序列含763个氨基酸,与奶牛、鸽及猪PRLR的同源性较高,其N末端含23个氨基酸的信号肽,成熟蛋白含789个氨基酸。gPRLR mRNA在成年鼠睾丸、输精管、卵巢、输卵管、肾、大肠、小肠、脾组织中均有表达。
The rat＇s prolactin receptor gene （gPRLR） cDNA with a length of 2743 bp was cloned by RACE -PCR, the result indicated that the eDNA contained 421 bp 5＇ - UTR, 1357 bp coding section and 218 bp 3＇ - UTR. As compared with ePRLR eDNA, （gPRLR） eDNA was presumably composed of 13 exons. The putative protein contained 763 amino acids, N - terminus of the protein comprised a signal peptide of 23 amino acids and the mature protein contained 789 amino acids. RT - PCR analysis showed that the gPRLR rnRNA was widely expressed in testis, seminiferous duct, kidney, ovary, oviduct, large intestine, small intestine and spleen.
出处
《江西农业学报》
CAS
2008年第12期3-5,共3页
Acta Agriculturae Jiangxi
