乳腺癌中生存素表达及其与Caspase-3和临床相关因素的关系

Expresstion of survivin in breast carcinoma and its relationship with Caspase-3 and clinical factors

目的探讨生存素(survivin)在乳腺癌表达及其与Caspase-3和临床相关因素的关系。方法采用免疫组化SP法检测生存素在正常乳腺组织(14例)、乳腺增生性病变(14例)、乳腺非浸润性癌(20例)和乳腺浸润性癌组织(52例)中的表达以及Caspase-3/CerbB-2/Ki67/p53/ER/PR在乳腺癌组织中的表达。结果生存素在这四组中阳性率分别为21.4%、35.7%、60.6%、71.2%(P<0.05)。淋巴结转移者生存素表达率高于无淋巴结转移者(P<0.05),生存素与Caspase-3在乳腺癌表达呈负相关(P<0.05);生存素表达阳性的乳腺癌Ki67/p53的标记指数(48.57±23.07)/(32.76±33.37)明显高于表达阴性的乳腺癌细胞Ki67的标记指数(21.30±20.50)/(15.43±24.44)(P<0.05)。结论生存素在乳腺癌进展中表达上调,提示可能通过与执行型Caspase-3结合,抑制其活性,从而阻止细胞凋亡,促进了细胞增殖与乳腺癌发生、发展;生存素有望成为乳腺癌诱导凋亡治疗的新靶点。

Objective To investigate the expression of survivin and its relationship with Caspase-3 and clinical factors in breast carcinoma. Methods Survivin and Caspase-3/CerbB-2/Ki67/ p53/ER/PR expressions were detected by immuohistochemisitry in the normal breast tissues, 14 cases） ,proliferative breast disease （epithelial hyperplasia, 14 cases）, breast preinvasive carcinoma （20 cases） and breast invasive carcinoma （52 cases）. Results The positiye rates of survivin expressin were 21.4%, 35.7%, 60.0% and 71.2% in the four groups, respectively （P〈 0.05）. Survivin expression was related to lymphnode metastasis （P〈 0. 05）. The expressin of Caspase-3 was negatively correlated with the expression of survivin （P〈 0. 05）. The Ki67/p53 indexes were significantly higher in survivin positive tumors （48. 57 ± 23. 07）/（32.76 ± 33. 37） than those in the negative ones （ 21.30 ± 20. 50）/（ 15.43 ± 24. 44） （P〈 0. 05）. Conclusion The overexpression of survivin may play an important role in the pathogenesis and progress of breast carcinoma by the way of inhibition of the active Caspase-a and of the accelerating proliferation. Survivin may be served as a new target for apoptosis-indueing therapy for breast carcinoma.