体内淋巴细胞微核试验在肺及肠癌患者术前检测中的意义

Significance of micronucleus test in vivo before therapy in patients with lung and colorectal cancers

目的探讨体内淋巴细胞微核形成与肠癌和肺癌患者肿瘤恶性程度的关系。方法随机抽取大肠癌、肺癌患者各一组,术前取外周血进行体内淋巴细胞微核试验检测,比较癌症患者体内自发微核形成与良性病变及健康人群对照组的差异。结果肺、大肠癌组微核率(MNF)与对照组相比,差异有统计学意义(P<0.01);随着患癌组分化程度降低及淋巴结转移率的增加,MNF明显上升。结论患者淋巴体内细胞微核形成与肺、大肠癌分化与转移密切相关,为癌者患者的术前恶性程度判断和高危人群筛查提供了一个有用的生物学标志物。

Objective To investigate the relationship of micronucleus （MN） formation in lymphocytes with the malignant degrees of cancers in patients with lung and colorectal cancers. Methods The MN test in vivo in capillary blood lymphoeytes was conducted in patients randomly selected from in-hospital patients before therapy. Experimental data were analyzed by comparing with frequency of MN between the patients with lung or colorectal cancers （experimental group） and those without cancer （control group）. Results The differences of the frequency of MN between the two groups were statistically significant （P〈0. 01）. The frequency of MN increased with the decrease of the histological differentiation and the increase of metastasis in experimental group. Conclusion The MN formation is significantly correlated with the malignant degree of cancers, and MN test will be a useful biomarker for screening of high-risk subjects and for identification of malignant degrees of eolorectal cancer before operation.