聚乳酸电纺构建仿生骨基质结构生物降解材料的体外细胞毒理研究

In vitro cytotoxicity of electrospun bioscaffolds with PLA that mimic nature structure of bone extracellular matrix

目的探讨通过聚乳酸静电纺丝的方法构建仿生骨基质多孔生物可降解材料的细胞毒性。方法采用MTT比色法检测4种浓度电纺聚乳酸细胞培养液的浸提液对人骨髓间充质细胞的增殖率影响,评价其细胞毒性。结果4种浓度的浸提液和阴性对照组的细胞增殖率之间无显著差异(P>0.05)。结论通过聚乳酸静电纺丝构建的仿生骨基质超微结构的生物降解材料无明显的细胞毒性,具有较好的生物相容性。

Objective To investigate the cytotoxicity of electrospun bioscaffolds with poly lactic acid （PLA） that mimic the nature structure of bone extracellular matrix in vitro. Methods The human bone mesenchymal cells were exposed to the treated cell culture medium for 7 d. Finally cell viability and growth were assessed by MTT assay. Results No significant cytotoxicity was observed among different concentrations and negative control Conclusions Electrospun bioseaffolds with PLA that mimic the nature structure of bone extracellular matrix doesn＇t demonstrate any significant toxic effects to human mesenchymal cells in vitro.