表面增强激光解析离子化蛋白芯片技术在重型颅脑创伤伤情判断中的应用

Application of surface enhanced laser desorption-ionization protein chip in judging injury severity of severe traumatic brain injury

目的探讨采用表面增强激光解析离子化（surface enhanced laser desorption-ionization，SELDI）蛋白芯片技术判断重型颅脑创伤（sTBI）患者伤情程度的可行性。方法利用弱阳离子芯片和质谱分析测定sTBI患者血清中的差异蛋白峰表达，分析峰强度与GCS评分的相关性。结果共获得101个有效蛋白质峰，其中27个峰间比较差异有统计学意义（P〈0．05），2个峰（m／z4972和m／z5322）强度与sTBI的GCS呈负相关，6个（m／z3941、m／z4295、rn／z8714、m／z8792、m／z14020和m／z28148）呈正相关。结论SELDI蛋白芯片技术可能成为未来sTBI患者伤情判断的客观检测方法之一。

Objective To judge injury severity of severe traumatic brain injury （sTBI） by using surface enhanced laser desorption-ionization （SELDI） protein chip technique. Methods Serum samples from sTBI patients were used to detect expression of differential proteins by protein chip CM10 and SELDI to analyze the correlation between expression peak intensity and GCS. Results We obtained 101 protein peaks, with statistical difference upon expression of 27 protein peaks, when negative correla- tion was found between two peaks （ m/z 4 972 and m/z 5 322） and GCS score and positive correlation between six peaks （m/z 3 941, m/z4 295, m/z 8 714, w/z 8 792, m/z 14 020 and m/z 28 148） and GCS score. Conclusion SELDI protein chip technique may become a new and objective detection method in judging injury severity of sTBI.