凋亡抑制蛋白livin在人皮肤鳞状细胞癌中表达的初步研究

The expression of livin in cutaneous squamous cell carcinoma

目的:研究凋亡抑制蛋白 livin 在人皮肤鳞状细胞癌(简称鳞癌)组织及细胞中的表达情况。方法:①采用免疫组化染色方法检测18例鳞癌组织中 livin 的表达情况;②利用反转录(RT)-PCR 及 Western blot 的方法检测皮肤鳞癌细胞系 A431细胞中 livin mRNA 及蛋白的表达情况。结果:①livin 蛋白在正常皮肤组织中不表达,鳞癌组织中 livin 蛋白的阳性表达率为72.2%;②livin 基因 mRNA 及蛋白在皮肤鳞癌细胞系 A431中表达较高。结论:凋亡抑制基因 livin 在人皮肤鳞癌组织及细胞中高表达,提示 livin 基因在皮肤鳞癌发生发展中起一定作用,为皮肤鳞癌的诊断及治疗研究提供实验基础。

Objective： To study the expression of livin, a recently described member of the inhibitor of apoptosis protein （lAP） family, in the cutaneous squamous cell carcinoma （SCC） and human SCC cell line. Methods： The immunohistoehemistry assay was used to detect the protein expression of livin in the specimens of 18 patients with SCC. Reverse transcription -polymerase chain reaction （ RT-PCR ） and Western blot assay were used to detect both the mRNA expression and the protein expression of livin in A431, a cell line of SCC. Results： The positive expression of livin protein was found in 72.2% of SCC specimens, which was not seen in the normal skin （P〈 0.01）. The higher mRNA and protein expression of livin was seen in A431, shown by RT-PCR and Westron blotting. Conclusions： There is a up-regulated expression of livin in cutaneous squamous cell carcinoma, which may play an important role in the carcinogenesis and development of it.