摘要
目的:研究凋亡抑制蛋白 livin 在人皮肤鳞状细胞癌(简称鳞癌)组织及细胞中的表达情况。方法:①采用免疫组化染色方法检测18例鳞癌组织中 livin 的表达情况;②利用反转录(RT)-PCR 及 Western blot 的方法检测皮肤鳞癌细胞系 A431细胞中 livin mRNA 及蛋白的表达情况。结果:①livin 蛋白在正常皮肤组织中不表达,鳞癌组织中 livin 蛋白的阳性表达率为72.2%;②livin 基因 mRNA 及蛋白在皮肤鳞癌细胞系 A431中表达较高。结论:凋亡抑制基因 livin 在人皮肤鳞癌组织及细胞中高表达,提示 livin 基因在皮肤鳞癌发生发展中起一定作用,为皮肤鳞癌的诊断及治疗研究提供实验基础。
Objective: To study the expression of livin, a recently described member of the inhibitor of apoptosis protein (lAP) family, in the cutaneous squamous cell carcinoma (SCC) and human SCC cell line. Methods: The immunohistoehemistry assay was used to detect the protein expression of livin in the specimens of 18 patients with SCC. Reverse transcription -polymerase chain reaction ( RT-PCR ) and Western blot assay were used to detect both the mRNA expression and the protein expression of livin in A431, a cell line of SCC. Results: The positive expression of livin protein was found in 72.2% of SCC specimens, which was not seen in the normal skin (P〈 0.01). The higher mRNA and protein expression of livin was seen in A431, shown by RT-PCR and Westron blotting. Conclusions: There is a up-regulated expression of livin in cutaneous squamous cell carcinoma, which may play an important role in the carcinogenesis and development of it.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2009年第1期15-17,共3页
Journal of Clinical Dermatology