葛根素对小鼠肠缺血再灌注肝损伤的保护作用

Protective Effects of Puerarin Injection on Liver Injury Induced by Intestinal Ischemia Reperfusion in Mice

目的:研究葛根素注射液对小鼠肠缺血再灌注所致肝损伤的保护作用。方法:按区组随机分组方法将小鼠分为模型组、假手术组及葛根素注射液(100,150,200 mg/kg)组,通过夹闭肠系膜上动脉建立肠缺血再灌注肝损伤模型。再灌注60 min后,取肝组织,测定肝组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)水平,光镜下观察肝组织形态学改变。结果:葛根素注射液可增强SOD活力,降低MDA水平,减轻肝组织形态学损伤。结论:葛根素注射液对小鼠肠缺血再灌注肝损伤具有一定的保护作用。

Objective： To investigate the protective effects of puerarin injection on intestinal ischemia reperfusion （I/R） induced injury of liver in mice. Methods： Mice were randomly divided into five groups as sham, model and puerarin injection （100,150 and 200 mg/kg） groups. Intestinal I/R models were established by clamping superior mesenterie artery for 20 min and reperfusing for 60 min. Then liver tissue samples were collected, then the SOD activity and content of MDA in liver tissue were determined. Histomorphological changes of liver tissue were observed with light microscope. Results： Puerarin injection could increase SOD activity, and decrease the content of MDA in liver tissue. Puerarin injection could attenuate liver tissue injury, as identified by microscopic examination of histomorphology. Conclusion： Puerarin injection can protect the liver of mice from injury induced by intestinal I/R.