荔枝核黄酮体外抗单纯疱疹病毒的作用

Antivirus Effects of Flavonoids Extracted from the Core of Litchi Chinensis Against Herpes Simplex Virus in vitro

目的:探讨荔枝核黄酮类提取物(FLC)体外抗单纯疱疹病毒1型(HSV-1)的作用。方法:以阿昔洛韦为阳性药物对照,以不同剂量的荔枝核黄酮类提取物分别作用于HSV-1病毒复制周期的各个环节分成3个实验组,以病毒半数感染量(TCID50)、细胞病变效应(CPE)、MTT染色细胞存活率(MTT法)作为评价指标,判断的FLC抗病毒效果。结果:FLC对HEp-2细胞的半数细胞毒浓度(TC50)为239.25 mg/L,FLC对HSV-1病毒有直接杀伤作用和抗HSV-1生物合成作用,其直接杀伤HSV-1的半数有效浓度(IC50)为32.76 mg/L,治疗指数(TI)为7.30,抗HSV-1生物合成作用的半数有效浓度(IC50)为12.72 mg/L,治疗指数(TI)为18.81。结论:荔枝核黄酮类化合物在HEp-2细胞中对单纯疱疹病毒1型有明显抑制作用。

Objective： To investigate the effect of flavonoids extracted from the core of Litchi chinensis （FLC） against herpes simplex virus type [ （HSV 1） in vitro. Methods： FLC was extracted by consulting the methods from correlative literature. According to viral replication cycle, three antiviral ways were designed to observe anti-HSV-1 effects of FLC by means of observing cytopathic effect （CPE） and adopting MTT colorimetric assay. And the Treatment Index （TI=TC50/IC50） was utilized as a comprehensive criteria to assess the effects of the extracts. In these experiments, acyclovir was used as the reference inhibitor. Results： Median toxic concentration （TC50） of FLC to HEp-2 cells was 239.25 mg/L. FLC was observed to directly inactivate HSV-1 and had anti biological synthesis in vitro, and its median inhibitory concentration （ICs0） of FLC anti- HSV 1 was 32.76 mg/L and 12.72 mg/Lrespectivety for its inactivating and anti-biological synthesis effect. The TI was 7.30 and 18.81 respectively. With the increasing of the dose, the de gree of CPE and viral titers in culture media decreased respectively, whereas viral inhibition rate increased. Conclusion： FLC had obviously direct inhibitory effects to HSV-1 in HEp-2 cells.