rhBMP-2/CPC对骨质疏松骨缺损的治疗作用的研究

Enhancement of bone defects healing by a local treatment with rhBMP-2/CPC in an osteoporotic goat

目的探讨磷酸钙骨水泥复合rhBMP-2对骨质疏松骨缺损的治疗作用。方法以卵巢切除法对10只成年山羊去势，低钙膳食6个月后建立骨质疏松模型；手术去除每只动物L2、L4椎体部分侧方骨质，造成约5mm×10mm×10mm大小骨缺损，将动物随机分成2组，一组植入rhBMP-2／CPC作为实验组，另一组植入CPC作为对照组；术后1周、9周分别采用双能X线吸收骨密度仪检测手术椎体骨密度、CT三维成像观察骨缺损愈合情况，术后9周手术椎体进行生物力学检测，制作椎体不脱钙切片进行骨组织计硅学分析。结果术后骨密度榆测实验组与对照组间差异无显著性，但实验组在骨缺损愈合状况、骨小梁微结构及骨牛物力学性能等方面均显著优于对照组。结论应用rhBMP-2／CPC能改善局部骨质疏松，促进骨质疏松骨缺损愈合，达到治疗骨质疏松骨折骨缺损的目的。

Objective To investigate the effect of a local treatment with recombinant human bone morphogenetic protein-2/calcium phosphate cement （rhBMP-2/CPC） in bone defects of an osteoporotic goat. Methods Ten mature goats underwent ovariectomy and were placed on low cation relative to anion diet. After six months, animals were randomly assigned to two groups （ the experimental group or the control group ）, and a bone defect with dimensions of 5 mm× 10 mm × 10 mm were made in L2 ,L4 of the goats. RhBMP-2/CPC or CPC were implanted into the defects of the experiment group or the control group respectively. After one or nine weeks, BMD were measured by dual energy X-ray absorption meter and healing of the defect was detected by CT scanning; Biomechanical test was applied to the vertebral body nine weeks later, micro architecture was also analyzed by bone histomorphometry. Results BMD had no difference between two groups, but bone defects of the experimental group healed more rapidly than the ones of the control group, and micro architecture and biomechanical property of the vertebral body were also improved in the experimental group. Conclusion Treatment with rhBMP-2/CPC can improve local osteoporosis and enhance the healing of an osteoporotic bone defect, so it may be used for the treatment of a bone defect or a fracture in osteoporosis.