QCM对牙釉基质蛋白在牙釉质表面的吸附行为

Study on adsorption of enamel matrix proteins onto enamel surface by QCM

目的:通过石英晶体微天平技术在牙釉质表面吸附牙釉基质蛋白,进而研究牙釉质表面对不同质量浓度蛋白吸附性能的影响。方法:在石英晶片上制备牙釉质膜,将其分别浸入10、20、40μg/mL质量浓度的牙釉基质蛋白溶液;通过共振频率、耗散因子、吸附量随质量浓度的变化关系,得出牙釉基质蛋白吸附的规律。结果:共振频率随质量浓度的提高而降低;吸附量随质量浓度的提高稳定的增长;结论:在20μg/mL的蛋白溶液中,牙釉质表面吸附蛋白的成膜刚性较好。石英晶体微天平技术对牙釉基质蛋白吸附机理的研究显得更直观,是研究蛋白吸附行为的有效工具。

Aim：The technology of quartz crystal microbalance （QCM） was applied to research on the adsorption behavior of enamel matrix proteins（EMPs） which are available on the enamel surface ,to study enamel matrix proteins on the impact of the enamel surface＇s adsorbable performance. Methods： The film of enamel was prepared for quartz wafer, soaked in a variety of EMPs concentrations ranging from 10,20, 40μg/mL; the adsorbed regularity of EMPs was acquired by the relation between the resonant frequency, energy dissipation, adsorbed mass and the various concentration. Results： The resonant frequency change decreased with the concentration, but the adsorbed amount increased steeply with the concentration. Conclusion：The adsorption at 20 μg/mL exhibited a much more viscose outer-layer structure. QCM which looks direct-viewing for the adsorbable mechanism of EMPs is an effective tool for study on the adsorbed behavior of protein.