松柏叶中槲皮素的提取及其含量测定

The Extraction and Determination of Quercetin in Several Plants of Pinaceae Lindl and Cupressaceae Bartling

采用超声波法提取了几种松柏科植物中的槲皮素,并用高效液相色谱法(HPLC)分别进行含量的测定.色谱条件如下:C18柱(250×4.6mm,5μm);流动相为乙腈与0.4%H3PO4的水溶液,比例为40:60;检测波长为360nm;流速为1.0mL·min-1.实验结果表明,松柏中都含有一定量的槲皮素,柏科植物中槲皮素的含量大约是松科植物的2～5倍,并且其槲皮素的含量随着科属的不同而不同.

In this paper, the quercetin was extracted by using ultrasonic in several plants of Pinaceae Lindl and Cupressaceae Bartling, the content of which was determined respectively by HPLC. Chromatographic conditions are as follows： Cl8 colum （250×4.6 mm, 5 μm） with acetonitrile-0.4% phocphroric acid of 40：60 as the mobile phase, the detection wavelength as 360 nm, the flow rate as 1.0 ml/min. The experimental results show that the plant of Pinaceae Lindl and Cupressaceae Bartling contain a certain amount of quercetin, which is about 2-5 times more than that of Cupressaceae Bartling, and that the content of quercetin is varied with the different genera. These will provide a new theoretical and reference basis for the development of medicinal plants and the study of genera identification for the plant of Pinaceae Lindl and Cupressaceae Bartling.