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食品副溶血性弧菌检验方法的合作研究 被引量:2

Cooperative Study of Method to Detect Vibrio Parahaemolyticus in Foods
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摘要 比较食品副溶血性弧菌检验国家标准GB/T4789.7-2003和修订法的检测效果。采用两种方法对3类食品(牛肉馅、冻鳕鱼和牡蛎肉)检测3个染菌水平,染菌试样共213份。结果显示,国标法和修订法对染菌试样的检测阳性数分别为47和83。对于3类食品的3个染菌水平,统计学检验结果提示,修订法的检测效果等同或优于国标法。 A multilaboratory study was conducted to compare the effectiveness of the GB method (GB/T 4789.7- 2003) and the revision method for detection of Vibrio parahaemolyticus in foods. Three food types (ground beef, frozen ling, and oyster meat) were analyzed by the two methods, and the total sample number was 213. For each food type, 3 inoculation levels were tested. The results showed that Vibrio parahaemolyticus was isolated from 47 and 83 samples using the GB method and the revision method, respectively. Based on the statistical results, for each of the 3 food types, at the 3 inoculation levels, the revision method performed was as well as or better than the GB method.
出处 《中国食品学报》 EI CAS CSCD 2008年第6期97-101,共5页 Journal of Chinese Institute Of Food Science and Technology
基金 "十一五"国家科技支撑计划(2006BAK02A15)
关键词 副溶血性弧菌 食品安全 食品卫生 检验 Vibrio parahaemolyticus Food Detection
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参考文献8

  • 1GB/T4789.7-2003食品卫生检验方法副溶血性弧菌检验[S].
  • 2Elliot EL, Kaysner CA, Jackson L, et al. Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp. [M]. FDA Bacteriological Analytical Manual[M]. Arlington:Association of Official Analytical Chemists,1995.
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同被引文献12

  • 1Ward LN, Bej AK. Detection of Vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes[J]. Appllied and Environmental Microbiology, 2006, 72(3): 2031-2042.
  • 2Tracz DM, Backhouse PG, Olson AB, et al. Rapid detection of Vibrio species using liquid microsphere arrays and real-time PCR targeting the ftsZ locus[J]. J Medical Microbiology, 2007, 56: 56-65.
  • 3Bushon RN, Kephart CM, Kohun GF, et al. Statistical assessment of DNA extraction reagent lot variability in real- time quantitative PCR[J]. Letters in Appllied Microbiology, 2010, 50(3): 276-82.
  • 4George M, Blackstone, Jessica L, et al. Detection of pathogenic Vibrio parahaemolyticus in oyster enrichments by real time PCR[J]. Journal of Microbiological Methods, 2003, 53(2): 149-155.
  • 5Kam KM, Lucy CK, Parsons MB, et al. Evaluation and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping Vibrio parahaemolyticus: an international muhicenter collaborative study[J]. Journal of Clinical Microbiology, 2008, 46(8): 2766-2773.
  • 6Blackstone GM, Nordstrom JL, Bowen MD,et al. Use of a real time PCR assay for detection of the ctxA gene of Vibrio cholerae in an environmental survey of Mobile Bay[J]. Journal of Microbiology Methods, 2007, 68(2): 254- 259.
  • 7Shuan JC, Lin TK, Tein NS. Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources [J]. The Journal of General and Appllied Microbiology, 2009, 55 (6): 419- 425.
  • 8Davis CR, HeUer LC, Peak KK, et al. Real-time PCR detection of the thermostable direct hemolysin and thermo- labile hemolysin genes in a Vibrio parahaemolyticus cultured from mussels and mussel homogenate associated with a foodborne outbreak[J]. Journal of Food Protection, 2004, 67(5): 1005-1008.
  • 9谢芝勋,谢丽基,庞耀珊,卢兆发,谢志勤,孙建华,邓显文,刘加波,唐小飞.WSSV和IHHNV二重实时荧光PCR检测方法的建立[J].水生生物学报,2009,33(1):22-27. 被引量:15
  • 10吴姗,吴志毅,张晓峰,黎昊雁.普通PCR法、荧光定量PCR法及微流控芯片法检测大豆产品中外源基因灵敏度的比较[J].中国食品学报,2009,9(2):176-186. 被引量:10

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