鮟鱇鱼皮硫酸皮肤素的二糖组成及结构研究

Studies on the Structure of Disaccharides in Dermatan Sulfate from Lophius Litulon Skin

目的:获取鮟鱇鱼皮中硫酸皮肤素的二糖结构信息。方法:以硫酸软骨素ABC酶酶解硫酸皮肤素样品,得到二糖;对酶解时间进行优化,进而以毛细管液相色谱法和毛细管液相-质谱联用法分析二糖结构及含量。结果:鮟鱇鱼皮硫酸皮肤素在硫酸软骨素ABC酶作用下,37℃反应4h,可酶解完全。其糖链是以艾杜糖醛酸-[1→3]-N-乙酰氨基半乳糖-4硫酸(△Di-4S(Ido))为主要二糖重复单位,此外,还含有少量的艾杜糖醛酸-[1→3]-N-乙酰氨基半乳糖-6硫酸(△Di-6S(Ido))和葡萄糖醛酸-[1→3]-N-乙酰氨基半乳糖(△Di-0S)二糖单位。△Di-4S(Ido)、△Di-0S与△Di-6S(Ido)3种不饱和二糖的摩尔比为171∶1∶1。

Objective： To obtain the information of the disaccharides in dermatan sulfate from Lophius litulon skin. Method： The DS sample was treated with chondroitinase ABC to afford disacchafides and the digesting time was optimized. CLC and CLC-MS method was used to analysis and determinate the disaccharide units content and structure in DS fragment. Result： The sample can be well digested at 37 ℃ for 4 hours treated with chondroitinase ABC. Polysaccha- ride chains of the DS consists of a prevailing disaccharide unit IdUA-[1→3]-GalNAc-4S（△Di-4S（Ido）） and some IdU- A-[1→3]-GalNAc-6S（ADi-6S（Ido）） and GlcUA-[1→3]-GalNAc（ADi-OS）, their mole ratio is 1.71：0.01：0.01.