茚三酮比色法测定野生及人工培养香菇和黑木耳中氨基酸含量

Content Determination of Amino Acid in Wild and Man-cultivated Lentinus Edodes and Auricularia auricular by Ninhydrin Colorimetric Method

目的:比较野生及人工培养香菇和黑木耳中氨基酸的含量,为高效、经济地开发利用资源提供依据。方法:采用茚三酮比色法测定氨基酸含量。结果:在60～100mg/L浓度范围内,浓度与吸光度呈良好线性关系。回归方程:A=0.0152C-0.792 8,r=0.9987,平均回收率为99.5%,RSD为0.06%(n=9)。野生及人工培养香菇中氨基酸的含量分别为14.54%、12.57%,野生及人工培养黑木耳中氨基酸的含量分别为8.83%、7.43%。结论:该方法操作简便,结果准确,重现性好。野生与人工培养的香菇和黑木耳中氨基酸含量没有显著差别。

Objective： To compare the content of amino acid in wild and man-cultivated Lentinus edodes and Auricularia auricular and to provide basis for the resource exploitation efficiently and economically. Methods： Ninhydrin Colorimetric Method was used to determine the content of amino acid. Results： From the range of 60-100mg/L, there was an excellent linear between the concentration and absorption. The regression equation was A=0.015 2C -0.792 8, r=0.998 7, the average recovery was 99.5% with RSD of 0.06% （n=9）. The contents were 14.54% and 12.57% for wild and man-cultivated Lentinus Edodes, 8.83% and 7.43% for Auricularia auricular respectively. Conclusion： The method was simple, accurate and repeatable. There were no significant differences of the contents in wild and man-cultivated Lentinus edodes and A uricularia auricular.