八羧酸酞菁钴配合物与牛血清白蛋白的结合作用研究

Studies on Octa-carboxylic Phthalocyanine Cobalt Interaction with Bovine Serum Albumin

八羧基金属酞菁的周边四个苯环各带有两个羧基,具有较好的水溶性,且吸收峰在670-700nm,是一类性能优良的光敏剂,而且CoPc(COOH)8带有负电荷,对蛋白有很强的亲和作用。采用光谱法研究了CoPc(COOH)8与牛血清白蛋白(BSA)的结合作用;采用荧光光谱分析方法测定了CoPc(COOH)8与BSA的结合常数为K=6.94×105;以氯血红素(HE)、布洛芬(IB)、L-色氨酸(TRP)为BSA的分子配体,络合竞争法研究了它们对CoPc(COOH)8-BSA体系紫外光谱的影响,并且确定了CoPc(COOH)8与BSA的结合点为SiteIII。

The interaction of octa-carboxylic phthalocyanine cobalt with bovine serum albumin （BSA） was studied. The binding constant KA of phthalocyanine cobalt was determined by both UV/Vis and fluorescence spectra methods. Besides, Hemin chloride（HE）,Ibuprofen（IB）and L-tryptophan（TRP） were used as ligands, the binding site of octa-carboxylic phthalocyanine cobalt on the BSA were found to be at the site Ⅲ by competitive binding method.