L-抗坏血酸紫外分光光度法检测微量过氧化氢

Use L-ascorbic Acid UV-spectrophotometry to Determine the Trace Hydrogen Peroxide

建立了L-抗坏血酸（L—Aseorbieacid）紫外分光光度法测定微量过氧化氢的新方法．在pH=5．7的磷酸缓冲溶液介质中，选择273nm作为测定波长，过氧化氢的摩尔浓度在0～0．1mmol／L范围内服从比尔定律．回归方程为：y=0．0049＋4．44612x（mmol／L），检出限为0．401μmol／L．该法用于溶液中、生物组织等样品中微量过氧化氢浓度的测定，快速简便．

A new method for determination of hydrogen peroxide in solution supply by UV - spectrophotometry was established. In a phosphate buffer medium of pH = 5.7 with an absorption wavelength 273nm, Beer,law is obeyed in the range of 0 - 0. l mmol/L for hydrogen peroxide and the linear regression equation is： y = 0. 0049 ＋ 4. 44612x（mmol/L） ;The detection limit of hydrogen peroxide is 0. 40lμmol/L. The method has been applied in rapid determination of hydrogen peroxide in some solution and organisms.