实时荧光定量PCR在登革热病毒快速检测中的应用

Rapid test of dengue virus by real-time quantitative PCR

目的应用实时荧光定量PCR快速检测登革热病毒感染。方法采集疑似登革热患者血清,采用实时荧光定量PCR检测登革热病毒,同时采用ELISA检测血清登革热IgM抗体。结果患者发病第7d血清登革热IgM血清抗体A值为0.236和0.237(临界值0.250),高度疑似阳性,第13d血清IgM抗体阳性。患者发病第2d,通用型核酸检测显示血清登革热病毒核酸含量较多,经过治疗,于第7d病毒拷贝下降。发病第2d,核酸检测确定感染病毒为登革热Ⅲ型;发病第13d血清登革Ⅲ型病毒核酸检测阴性。结论实时荧光定量PCR法具有准确性好、灵敏度高等优点,可用于登革热病毒感染的快速检测。

Objective To test dengue virus by real-time quantitative PCR. Methods The serum of suspect patient with dengue virus infection was collected. Dengue virus RNA was detected by real-time PCR assay and its serum IgM antibody was measured by enzyme linked immunosorbent assay （ELISA）. Results The A value of suspect patient＇s serum antibody in seventh day was 0.236 and 0.237（the critical value was 0.250）, and the serum IgM antibody of patient was positive in thirteenth day. A large number of dengue virus RNA was detected in the second day after onset of clinical symptoms. The mRNA expression level decreased after treatment. Dengue Ⅲ virus RNA was detected in the second day by real-time quantitative PCR, but negative in the thirteenth day. Conclusion Real-time fluorescent PCR was an ideal method for rapid dengue virus infection test with high accuracy and sensitivity.