微小隐孢子虫重组BCG-CP15/60-23疫苗免疫原性的研究

Immunogenicity of recombinant BCG expressing Cryptosporidium parvum CP15/60-23 antigen gene

目的探讨微小隐孢子虫重组BCG-CP15/60-23疫苗(rBCG-CP15/60-23)的免疫原性。方法用rBCG-CP15/60-23免疫BALB/c小鼠,免疫前后取血,用ELISA法检测IgG抗体,末次免疫后2周取淋巴细胞作体外培养,测定培养上清IFN-γ、IL-2和IL-12的水平。设实验组、BCG组、载体组和PBS对照组。结果rBCG-CP15/60-23免疫后2周开始小鼠IgG抗体水平逐渐升高,与BCG组和载体组比较差异均有统计学意义(P均<0.01);免疫鼠脾淋巴细胞培养上清中IFN-γ和IL-2的含量与PBS对照组和BCG组比较差异有统计学意义(P均<0.05),IL-12水平组间差异无统计学意义(P>0.05)。结论rBCG-CP15/60-23免疫小鼠后能刺激机体产生IgG抗体,IFN-γ和IL-2的分泌增加,具有较好的免疫原性。

Objective To study the protective effect of recombinant BCG-CP15/60-23 vaccine for Cryptosporidium parvum （rBCG-CP15/60-23）. Methods The mice in the experimental groups were injected into vena caudalis with rBCG-CP15/60-23. IgG in serum and cytokines IFN-γ, IL-2 and IL-12 in supernatant of splenocytes were detected using ELISA. Results The level of IgG of immunized mice set up gradually after 2 weeks from immunifaction, there was significant difference compared with the BCG group and vector group（P〈0.01）, the splenocytes produce more IFN-γ and IL-2 in experimental groups than control and BCG groups（P〈0.05）, but there was no statistical differenc of IL-12（P〉0.05）. Conclusion The results indicated that the rBCG-CP15/60-23 can induce IgG antibody and induce the blastogenisis of immunized splenocytes to produce IFN-γ and IL-2 in vitro and immunogenicity.