心脏骤停大鼠复苏后心脑内IL-1β和TNF-α的变化

Changes of tumor necrosis factor-α,interleukin-1β in the brain and cardia of cardiac arrest resuscitation rats

目的探讨窒息至心脏骤停大鼠复苏后心脑IL-1β和TNF-α的变化。方法采用呼气末夹闭气管窒息法建立大鼠心脏骤停模型,并心肺复苏成功后生存24 h,将20只健康Wistar大鼠随机2组分别为模型组,正常对照组。复苏24 h后处死大鼠,取心、脑组织标本,电镜下观察心、脑组织的病理改变。采用酶联免疫吸附法(ELISA)检测心、脑组织中IL-1β和TNF-α的含量变化。结果心、脑组织病理观察显示,与对照组相比模型组心脑细胞超微结构出现损伤性改变;心、脑中IL-1β和TNF-α含量变化,与对照组相比模型组心、脑IL-1β和TNF-α含量明显升高。结论心跳骤停复苏后大鼠心、脑组织内IL-1β和TNF-α等炎症细胞因子含量增多,提示心脑内存在着炎症介质泛滥,失控,并加重心脑损伤。

Objective To observe changes of cytokine tumor necrosis factor-α（TNF-α）, interleukin-16（IL-16） after cardiopulmonary cerebral resuscitation rats.Methods The animal model of cardiac arrest（CA） was made by clamping endotracheal tube at expiration , and exist twent-four hours after cardiopulmonary resuscitation（CPR）, twenty healthy Wistar rats were randomly divided into control group,model group.Kill the rats after twenty-fours hours,The cortex of the brain and the heart was taken out immediately to observe the ultrastructure changes.The levels of TNF-α and IL-1β were determined by ELISA.Results Compare with contral group the ultrstmeture of brain and heart were ,damodged in model group,compare with eontral group the level of IL-1β TNF-α were increase significantly in the brain and heart of model group. Conchusion Quanfitation of TNF-α and IL-1β expression from cardiopulmonary cerebral resuscitation rats increase; The mediators of inflarnmation overflow in cardia and brain of the rats, and aggravate damage of cardia and brain.