乳酸脱氢酶同工酶1(LD1)在诊断儿童心肌细胞损伤中的应用

The Application of lactate dehydrogenase isoenzymes in diagnsis of children's myocardial cell injury

目的探讨LD1在辅助诊断儿童心肌细胞损伤中的应用价值。方法用日立7180全自动化分析仪对594例门诊的儿科患者(年龄<=14岁)同时检测心肌酶谱:磷酸肌酸激酶(CK)、磷酸肌酸激酶MB同工酶(CK-MB)、乳酸脱氢酶(LD)、乳酸脱氢酶同工酶1(LD1)、天门冬氨酸转移梅(AST)、α-羟丁酸脱氢酶(-αHBD),比较各项检测指标对心肌细胞损伤的诊断价值。结果对594例患者心肌酶谱检测中,伴有CK升高的占2.5%,CK-MB升高的占39.9%,LD升高的占66.2%,-αHBD升高的占63.0%,AST升高的占9.4%,LD1升高的占1.0%,心肌细胞损伤诊断4例,占0.6%。结论将LD1组合到心肌酶谱的检测中,可有效排除非心肌细胞损伤引起的传统心肌酶谱中检测结果的非特异性增高,对心肌细胞损伤的筛查有较好的应用价值。

Objecfive to investigate the practical value of LD1 in the diagnosis of children＇s myocardial cell injury.Methods to test myocardial enzyme （CK, CK-MB,LD,LDI,AST, α-HBD） of 594 pediatric outpatients（ age： 〈 = 14） simultancously with Hitachi 7180 Automatic Biochemistry Analyzer and to compare the diagnostic value of the different test indexes in the diagnosis of myocardial cell injury. Results The results are as follows：the samples with CK increase make up 2.5 % ;those with CK-MB increase make up39.9% ;those with LD increase constitute 66.2% ;those with a-HBD increase constitute 63.0% ;those with AST increase account for 9.4% while those with LD1 increase account for 1.0% .Four cases are diagnosed to be myocardial injury,making up 0. 6%. Conclusion To include LD1 in myocardial enzyme test can effectively help to eliminate the nonspecific increase of the test indexes in the traditional myocardial enzyme test,which is caused by nonmyocardial cell injury;therefore,it has a good practical value in the screening of myocardial cell injury.