摘要
目的探讨可溶性细胞黏附分子-1(sICAM-1)的含量在乙型肝炎患者纤维化病程中的表达及意义。方法选择80例处在非急性发作期的乙型肝炎患者进行肝组织穿刺检测,分成三组,选取30例健康体检者作为对照组。上述四组均空腹抽取静脉血,留取血清使用酶联免疫吸附法(ELISA)检测各组血清中的sICAM-1含量;同时,采用放射免疫分析法检测纤维化四项包括血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前妥胶原(PCⅢ)、Ⅳ型胶原蛋白(Ⅳ.C)含量。结果sICAM-1在乙肝患者的血清含量随着肝纤维化严重程度呈阶梯样上升,有显著性差异(P<0.05)。各组血清sICAM-1含量与肝纤维化指标HA、LN、Ⅳ.C呈正相关(P<0.01)。结论sICAM-1可作为判断肝纤维化的血清学指标,动态检测肝纤维化进程。而且,sICAM-1也可以为临床肝纤维化检测提供非创伤性的早期诊断依据。
Objective To evaluate the sICAM-1 serum level of liver fibrosis in hepatitis B and its clinical significance. Methods The sample of liver biopsy was obtained from 80 patients with hepatitis B which were not in the acute disease developmental stage; the patients were divided into three groups to the child grade with liver fibrosis.At the same time,the expression of sICAM-1 in serum of three groups and 30 cases of normal subjects were detected by using the techniques of ELISA; hyaluronic acid （HA） .laminin（LN）. procollagen type Ⅲ （PCⅢ） .type Ⅳ procollagen （ Ⅳ. C） were also detected by radio immunity analysis method.Results The serum levels of sICAM- 1 of patients with hepatitis B increased significantly with the severity of liver fibrosis （ P 〈 0.05）. The sermn levels of sICAM-1 was positively eorrelated with the serum levels of HA , LN , Ⅳ . C （P〈0.01）.Condusion The serum levels of sICAM-1 can be used to monitor the liver fibrosis and also be helpful the early diagnosis.
出处
《中国实验诊断学》
北大核心
2009年第1期88-90,F0003,共4页
Chinese Journal of Laboratory Diagnosis
