慢性淋巴细胞白血病患者孕激素受体B基因甲基化异常的检测

Detecting the aberent methylation of progesterone receptor B gene in human chronic lymphocytic leukemia.

目的探讨检测慢性淋巴细胞白血病患者孕激素受体B基因CpG岛甲基化状态改变的临床意义。方法提取外周血DNA,经亚硫酸盐修饰,然后采用甲基化特异性PCR技术检测9例慢性淋巴细胞白血病患者及5例正常对照外周血DNA孕激素受体B基因CpG岛甲基化状态。结果在9例慢性淋巴细胞白血病患者外周血中5例成功地检测到孕激素受体B基因甲基化异常,而正常对照均没有发现甲基化异常。结论对慢淋孕激素受体B基因异常甲基化的检测该方法可能对监测肿瘤进程有一定作用。

Objective To study the clinical value of detecting the methylation status of progesterone receptor B gene in chronic lym- phocytic leukemia patients. Methods： DNA was isolated from peripheral blood and treated with Sodium Bisulfite, then analysed by methylation -specific polymerase chain reaction （MSP）. Methods： Nine samples from patients and five controls were analysed. Resuits ： PR - B gene showed aberrant methylations in 5 samples from patients, but none in normal controls. Conclusion ： We set up a reliable method to detect PR - B gene aberrant methlation, and it might be useful for monitoring tumor progreggen.