鸦胆子油对大鼠增生型子宫内膜血管内皮生长因子表达的影响

Influence of VEGF of brucea javanica oil on proliferative endometrium hyperplasia of rats

目的观察不同浓度鸦胆子油对大鼠增生型子宫内膜血管内皮生长因子(VEGF)表达的影响及意义,探讨鸦胆子油治疗功能失调性子宫出血的分子生物学机制。方法建立大鼠增生型子宫内膜模型,设正常对照组(A)、模型对照组(B)、鸦胆子油宫腔注射低(C)中(D)高(E)剂量组并进行相应处理,用药结束后进行免疫组化法检测各组VEGF的表达及意义。结果各组VEGF表达情况:A组阳性表达,B组强阳性表达,C组弱阳性表达,D组腺体结构不清,E组无腺体结构。A组与B组有显著性差异(P<0.05),B组与C组之间及A组与C组之间有极显著差异(P均<0.01)。结论雌激素促使去卵巢大鼠子宫内膜增厚、腺体增生、子宫内膜腺上皮细胞VEGF表达增强。鸦胆子油靶向作用大鼠增殖型子宫内膜,破坏腺上皮细胞组织结构,影响其分泌VEGF,致腺上皮细胞VEGF表达降低,进而影响内膜血管形成。

Objective It is to observe the influence and significance of the brucea javanica oil on proliferative endometrium of rats, in order to discuss the molecular organism mechanism of brucea javanica oil in treating dysfunctional uterine bleeding. Methods The rats were divided into five groups, they are the normal contrasting group（A）, the model group（B）, the lower dosage group of brucea javanica oil （C）, the medium dosage group of brucea javanica oil（D）, the higher dosage group of brucea javanica oil （E）. The models of proliferative endometrium of rats were established, and the rats were treated accordingly in all groups. The expressions of VEGF were detected by immunohistochemical SP staining in all groups. Results The expression of VEGF is masccline in group A, and strong masccline in group B, and weak masccline in group C, and the gland structure was unclear in group D, and there was no gland structure in group E. The difference of the expression of VEGF was significant between A and B group （P 〈 0.05）, and it were extremely significant between group B and C, A and C separately （P 〈 0.01 ）. Conclusion Estrogen makes the endometrium thicken, the glands hyperplasy, the VEGF in endometrium glandular epithelium strong express in rats without ovaries. The brucea javanica oil targetting proliferative endometrium, destroy the structure of glandular epithelium cell, affect the excretion of VEGF, decreases VEGF expression in glandular epithelium. So does the formation of endometrium blood vessel.