BPA与3种双酚类化合物的联合作用雌激素效应

Jointed Estrogenic Activities of Bisphenol A and Three of Its Analogs

环境内分泌干扰物的联合作用已经是目前研究的重点.采用重组基因酵母检测法,分别测定了双酚A(BPA)、双酚AF(BPAF)、双酚AP(BPAP)、双酚F(BPF)4种双酚化合物的雌激素活性,并依据其测定结果,按照等效浓度比,设计了浓度比分别为EC10和EC50的6种二元混合物并测定了其雌激素活性.结果表明,这4种化合物的剂量-效应关系都可以用Weibull函数有效描述,BPA、BPAF、BPAP、BPF的EC50值分别为:6.81×10-6、7.44×10-7、1.43×10-5、7.52×10-6mol.L-1,其雌激素活性大小顺序为:BPAF>BPA>BPF>BPAP.依据不同的联合作用判断方法对这4种化合物的联合效应进行判断,结果表明,相同化合物的不同混合比例可能对联合作用方式产生影响,采用DA和IA预测模型可以更加直观、方便地判断联合作用的类型,而且可以反映出不同混合比例对联合作用的影响.

The combined effect of environmental endocrine disruptors（EEDs） is one of the hottest topics. The estrogenic activities of BPA, BPAF, BPAP, BPF were tested based on recombinant gene yeast assay. Six mixtures were designed based on the result of the test,each of which had an equitoxic ratio ray （EC10 or EC50）.The ECso values are 6.81× 10^-6mol·L^-1,7.44× 10^-7 mol·L^-1,1.43× 10^-5 mol·L^-1,7.52× 10^- 6 mol·L^-1 for BPA, BPAF, BPAP and BPF respectively, which reveals that the estrogenic activities order among the four bisphenols was BPAF 〉 BPA 〉 BPF 〉 BPAP. The experiment shows that when BPA mixes with BPAF, BPAP and BPF in different ratios individually, different combination effects are produced. It reveals that the combined ratios of the components may affect the combined effect. The dose addition model and the independent action model are used to identify the combined effect. They are testified to be more intuitionistic and more comprehensive than other joint effect indices.