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轮状病毒VP7基因的克隆及其植物表达载体的构建 被引量：1

Cloning and Construction of Plant Expression Vector Containing Rotavirus VP7 Gene
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摘要 应用聚合酶链式反应技术(PCR)扩增了轮状病毒VP7基因,并将其克隆到pMD18-Tvector载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了DNA全序列。结果表明,克隆片段全长为981bp。将轮状病毒VP7基因定向的克隆到植物表达载体pBI121启动子下游,构建了植物表达载体。 Rotavirus VP7 gene was obtained by PCR techinque and cloned into pMD18-Tvector. The recombinant clone was detected by PCR technique and analyzed by the restriction enzyme. A full length of cDNA gene was sequenced. The results showed that the length of the clone sequence was 981bp. Rotavirus VP7 gene was cloned into promoter downstream of plant expression vector pBI121 in orientation.
作者 柴晓杰 靳非 王宇航 王晓庆
机构地区 大连水产学院生命科学与技术学院
出处 《生物技术通报》 CAS CSCD 北大核心 2009年第1期80-82,共3页 Biotechnology Bulletin
基金 博士基金(20040219)
关键词 轮状病毒 PCR 克隆 植物表达载体 Rotavirus PCR Clone Plant expression vector
分类号 Q939.4 [生物学—微生物学] Q943.2 [生物学—植物学]
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参考文献12

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生物技术通报
2009年 第1期

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